Purpose
CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
Inserts
Decoy sgRNA
dCas9
Chloramphenicol acetyltransferase
Use
CRISPR; Toxoplasma gondii expression
Tags
3X FLAG and Nuclear Localization Signal
Promoter
TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
Available Since
Oct. 4, 2021
Availability
Academic Institutions and Nonprofits only