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pJC45BS104 L892W

Plasmid

#1308

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L892W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 892 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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ZE36 Nlk1(C387Y)-pCS2+

Plasmid

#17077

Depositor

Randall Moon

Article

Thorpe et al Development. 2004 Jun;131(12):2899-909.

Insert

Nlk1 (nlk1 Zebrafish)

Use

Zebrafish expression

Mutation

C387Y. Inactive mutant of NLK1.

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pJC45BS104 F130W

Plasmid

#1302

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 F130W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Phe 130 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-ora

Plasmid

#20027

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pJC45BS104 L553W

Plasmid

#1306

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L553W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 553 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pEM110

Plasmid

#250586

Purpose

H2Apr-MRLC-mClover3-FLAG-MRLCter

Depositor

Lillian Fritz-Laylin

Article

Medina et al Curr Biol. 2026 Jan 19;36(2):338-354.e5.

Insert

H2Bpr-P2A-T2A-HygR-Synther8:H2Apr-SpunMLRC-mClover3-SpunMLRCter

Use

Agrobacterium binary plasmid

Tags

mClover3

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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LIMK1-s

Plasmid

#245963

Purpose

Expression of inactive LIMK-1 with some dominant negative acivity and GFP from separate CMV promoter

Depositor

James Bamburg

Insert

LIM domain kinase 1 (Limk2 Mouse)

Expression

Mammalian

Mutation

deletion of 20 amino acids from kinase domain

Promoter

CMV for LIMK and separate CMV for eGFP

Available Since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPBpuro-RasS17N

Plasmid

#214820

Purpose

Encoding dominant negative HRas

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

Dominant negative HRas

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-Rac1T17N

Plasmid

#214821

Purpose

Encoding dominant negative Rac1

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

Dominant negative Rac1

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-Rab5S34N

Plasmid

#214822

Purpose

Encoding dominant negative Rab5A

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

Dominant negative Rab5

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pJT87

Plasmid

#204489

Purpose

CAG promoted WBeta recombinase

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Insert

WBeta recombinase

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pJT88

Plasmid

#204490

Purpose

CAG promoted phiC31 recombinase

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Insert

phiC31 recombinase

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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CSII-mApple

Plasmid

#207350

Purpose

Mammalian expression of mApple

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

mApple

Expression

Mammalian

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pATT-Dest-Cre

Plasmid

#202714

Purpose

Cre recombinase staging plasmid to make transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

Cre

Use

Staging plasmid to make transposon insertion libr…

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPEPT1

Plasmid

#195001

Purpose

Production of peptides in E. coli as GB1 fusion

Depositor

Marko Hyvönen

Article

Pantelejevs et al Chem Sci. 2023 Nov 21;14(47):13915-13923

Type

Empty backbone

Tags

His8-tag and StrepTagII-GB1

Expression

Bacterial

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB9360(IX-1_Markerfree_BackBone)

Plasmid

#161601

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site IX-1, (Chr IX: 388864..388883)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9362(XV-1_Markerfree_BackBone)

Plasmid

#161603

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XV-1, (Chr XV: 93186..93205)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9355(II-1_Markerfree_BackBone)

Plasmid

#161597

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site II-1, (Chr II: 341622..341641)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9356(IV-1_Markerfree_BackBone)

Plasmid

#161598

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site IV-1, (Chr IV: 132921..132940)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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C453-E26: attB4-T7p>-attB5

Plasmid

#162982

Purpose

Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

bacteriophage T7 promoter

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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