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Hmga2 3'UTR wt luciferase (Luc-wt)

Plasmid

#14785

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Luc-wt (Hmga2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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ACPPiso1-V5

Plasmid

#113052

Purpose

Expresses human prostatic acid phosphatase (isoform 1) in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

Insert

prostatic acid phosphatase isoform 1 (ACP3 Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pME-lamp1

Plasmid

#109559

Purpose

Multisite gateway vector for 5' tagging with lamp1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

lamp1 (lamp1b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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ACPPiso2-V5

Plasmid

#113053

Purpose

Expresses human prostatic acid phosphatase (isoform 2) in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

Insert

prostatic acid phosphatase isoform 2 (ACP3 Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI

Plasmid

#115270

Purpose

Expresses TtCsm6 from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

TtCsm6

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Erk2

Plasmid

#29582

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Erk2 (Mapk1 Rat)

Tags

HIS6 and TEV

Expression

Bacterial

Mutation

Starting Met removed from Erk2

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only

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HsSPHK1

Plasmid

#118091

Purpose

Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

Depositor

Kevin Lynch

Article

Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

Insert

Homo sapiens Sphingosine Kinase 1

Tags

FLAG

Expression

Yeast

Promoter

GAL1, 10

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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RhoA

Plasmid

#55699

Purpose

expression of mouse RhoA

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoA (Rhoa Mouse)

Tags

GST

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP3-EGFP

Plasmid

#168504

Purpose

Expression of full length SRGAP3 with a C-terminal EGFP fusion

Depositor

Franck Polleux

Article

Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

Insert

SRGAP3 (SRGAP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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human p53-(355-393)

Plasmid

#24870

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains aa 355-393.

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
pIVT-PuroR

Plasmid

#232753

Purpose

IVT template for making modRNA encoding the Puromycin resistance gene

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

PuroR

Use

In vitro transcription

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab13 in pCSDEST2

Plasmid

#140879

Purpose

Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab13 (rab13 Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-PAmCherry1-MCS

Plasmid

#60608

Purpose

Cloning PAmCherry1

Depositor

Steven Chu, Xiaolin Nan

Article

Nan et al Proc Natl Acad Sci U S A. 2013 Nov 12;11

Insert

PAmCherry1

Use

Cloning

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pJM005

Plasmid

#134150

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAC025

Plasmid

#227597

Purpose

Lentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motif

Depositor

Brittany Adamson

Article

Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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PT7-deGFP-MGapt

Plasmid

#67741

Purpose

T7 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T7term

Use

Synthetic Biology

Expression

Bacterial

Promoter

PT7

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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mSIRT3-L

Plasmid

#33309

Depositor

Michael Sack

Article

Bao et al J Cell Biochem. 2010 May;110(1):238-47.

Insert

Sirt3 (Sirt3 Mouse)

Tags

3XFlag

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-oA ONE-GO

Plasmid

#204335

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-oA ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Flag-FOXB1

Plasmid

#153112

Purpose

Expression of Flag tagged human FOXB1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXB1 (FOXB1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pT3TS_Transposase

Plasmid

#109768

Purpose

Codon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Tol2 Transposase

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Nck

Plasmid

#186133

Purpose

For expression of recombinant Nck in bacteria cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

NCK1 (NCK1 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acid residues 1-377, with mutations: C139S,…

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-pre-eGRASP(p30)

Plasmid

#111597

Purpose

An AAV vector that expresses green pre-eGRASP(p30) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

green pre-eGRASP(p30)

Use

AAV

Promoter

CaMKIIa

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pJM010

Plasmid

#134154

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_KMLL (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

KMLL

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt2

Plasmid

#184503

Purpose

Mammalian expression of Synaptotagmin 2 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 2 (Syt2 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRG706_lexO-SbfI_LexA-TF_LEU2MX

Plasmid

#154818

Purpose

Inducible expression of SbfI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

SbfI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTREX GPR68_NB2_2

Plasmid

#127691

Purpose

Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

GPR68_NB2_2

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 5HT2A_NB2_1

Plasmid

#127690

Purpose

Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

5HT2A_NB2

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-3aHA

Plasmid

#18030

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Tags

HA

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

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actc1b-Lifeact-mKate2

Plasmid

#109493

Purpose

Muscle specific zebrafish expression of mKate2 fused to the actin binding lifeact sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-Lifeact-mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXB2

Plasmid

#153113

Purpose

Expression of Flag tagged human FOXB2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXB2 (FOXB2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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