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We narrowed to 17,391 results for: puro

Showing: 8501 - 8550 of 17391 results

  6. pHJ18

    Plasmid
    #48357
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48357-plasmid-map-sequence-id-70046

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  16. Halo-ATG16L1 HRD

    Plasmid
    #207537
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    HaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    207537-plasmid-map-sequence-id-470412

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  17. Halo-MDC1 HRD

    Plasmid
    #207086
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous MDC1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human MDC1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207086-plasmid-map-sequence-id-431126

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  18. RNF169-Halo HRD

    Plasmid
    #207083
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207083-plasmid-map-sequence-id-432319

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  19. SNAP-LC3 HRD

    Plasmid
    #207545
    Purpose
    Homologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    SNAPtag with internal PuroR cassette flanked by human LC3B locus sequences
    Use
    Tags
    SNAPtag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    207545-plasmid-map-sequence-id-430987

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  20. Halo-LC3B HRD

    Plasmid
    #207539
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    HaloTag with internal PuroR cassette flanked by human LC3B locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    207539-plasmid-map-sequence-id-429514

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  23. pHJ33

    Plasmid
    #48362
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48362-plasmid-map-sequence-id-70053

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  24. AAVS1-tdTomato

    Plasmid
    #159275
    Purpose
    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Article
    Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11
    Insert
    tdTomato
    Use
    AAV; S1 knockin donor vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159275-plasmid-map-sequence-id-312908

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  25. HypoxCR

    Plasmid
    #59946
    Purpose
    A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.
    Depositor
    Anne Le
    Article
    Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p
    Inserts
    GFP-Pest
    mCherry-Geminin
    Use
    Lentiviral
    Tags
    FLAG and PEST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and min CMV
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    59946-plasmid-map-sequence-id-228094

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  30. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

    Plasmid
    #175508
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175508-plasmid-map-sequence-id-345097

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  31. AD59_pEP.DonorCLYBL.TS

    Plasmid
    #199227
    Purpose
    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.
    Insert
    Expression unit for PuroR.T2A.EGFP selectable and reporter markers
    Use
    Gene targeting donor plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human PGK1 gene promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199227-plasmid-map-sequence-id-391392

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  32. PB-CT3G-CERP2-MG-IRES2-mTagBFP2

    Plasmid
    #175509
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175509-plasmid-map-sequence-id-345099

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  37. Halo-TCAB1 HRD

    Plasmid
    #207535
    Purpose
    Homologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.
    Depositor
    Jens Schmidt
    Article
    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:
    Insert
    HaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207535-plasmid-map-sequence-id-430972

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  38. pCLucIPZ

    Plasmid
    #53222
    Purpose
    Constitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interest
    Depositor
    Thorsten Stiewe
    Article
    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1
    Inserts
    Cypridina luciferase
    non-silencing shRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    53222-plasmid-map-sequence-id-342548

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  39. ULK1-Halo HRD

    Plasmid
    #207548
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    207548-plasmid-map-sequence-id-430489

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  40. PB-CT3G-CERP2-MG-IRES2-mCherry

    Plasmid
    #175507
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    175507-plasmid-map-sequence-id-345876

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  41. pHDR-USR-eGFP

    Plasmid
    #208830
    Purpose
    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.
    Depositor
    Zhiying Zhang
    Article
    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    208830-plasmid-map-sequence-id-414896

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  45. pL-SFFV.Reporter.RFP657.PAC

    Plasmid
    #61395
    Purpose
    Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.
    Depositor
    Benjamin Ebert
    Article
    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61395-plasmid-map-sequence-id-101662

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  48. pGLucIPZ

    Plasmid
    #228703
    Purpose
    Constitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interest
    Depositor
    Thorsten Stiewe
    Article
    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1
    Inserts
    Gaussia luciferase
    non-silencing shRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    228703-plasmid-map-sequence-id-454814

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Showing: 8501 - 8550 of 17391 results