We narrowed to 170,492 results for: addgene

Showing: 3401 - 3420 of 170492 results

Results per page:

Sort results by:

LentiCRISPRv2-scr

Plasmid

#169795

Purpose

Expresses Cas9 and guide RNA with untarget sequence generated by scramble of the target sequence of LentiCRISPRv2-ACTB-C1..

Depositor

Natsuko Chiba

Article

Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

Insert

Scrambled guide RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HLA_DRA_01_01

Plasmid

#165012

Purpose

Expression of HLA-DRA*01:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRA (HLA-DRA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CROPseq-Puro-4xMS2

Plasmid

#212626

Purpose

MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-FoxO1-Clover

Plasmid

#67759

Purpose

Fluorescent fusion protein for tracking Akt signaling

Depositor

Peter Rotwein

Article

Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

Insert

Fox01 (Foxo1 Mouse)

Use

Lentiviral

Tags

Clover

Mutation

Changed Serine 209 to Alanine, changed Histidine …

Promoter

EF1a

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 blast hPGK

Plasmid

#206229

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cyto-V5-APEX2

Plasmid

#170572

Purpose

Expresses APEX2 in cytosol

Depositor

James Olzmann

Article

Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

Insert

APEX2

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH335_MS2-AID*Δ-Hygro

Plasmid

#85406

Purpose

lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID*Δ and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Marathon-RT for Split-PE (pEH59)

Plasmid

#190108

Purpose

Expresses Marathon-RT (human codon optimization)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

Marathon-RT

Tags

bpNLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-eGFP-dSaCas9-blast

Plasmid

#191653

Purpose

Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

eGFP-T2A-dSaCas9

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX301-ZIM3-KRAB-PYL1

Plasmid

#154761

Purpose

Expresses ZIM3 KRAB domain fused to PYL1

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

CMV

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Punc-4c.TIR1

Plasmid

#194053

Purpose

TIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegans

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

TIR1 (TIR1 Mustard Weed)

Expression

Worm

Mutation

codon-optimized for expression in C. elegans; Two…

Promoter

unc-4c

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pROSA26-PA

Plasmid

#21271

Depositor

Frank Costantini

Article

Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

Insert

ROSA26 (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pSQT1313

Plasmid

#53370

Purpose

multiplex gRNA expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

None

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRG757-smedwi-1

Plasmid

#99074

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Smedwi-1

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Smedwi-1 in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK DN RhoA

Plasmid

#84646

Purpose

Lentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of Venus

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

RhoA (RHOA Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Dominant Negative

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only
pRRG227-Wnt1

Plasmid

#99073

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Wnt1 (also known as WntP-1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Wnt1 (also known as WntP-1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-Flag-CtIP

Plasmid

#71108

Purpose

mammalian expession of Flag-CtlP

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Please see Depositor Comments

Promoter

TRE promoter, Tet ON

Available Since

Nov. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pRRG217-soxB

Plasmid

#99086

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea soxB (also known as soxB1-1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

soxB (also known as soxB1-1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BPK3082

Plasmid

#78742

Purpose

Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

LbCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
BAC-GWS1B

Plasmid

#239581

Purpose

Single copy expression vector for GWS1B Rubisco

Depositor

Matthew D. Shoulders

Article

McDonald et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

GWS1B Rubisco (6X his)

Tags

6X histag

Expression

Bacterial

Mutation

Wild-type

Promoter

T7, tac

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge