We narrowed to 4,074 results for: p2a

1. pY30

   Plasmid

   #84745

   Purpose

   Expresses huAsCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   puromycin resistance gene
   

   Use

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSK+ chickBMPR-1B CA

   Plasmid

   #49528

   Purpose

   cloning vector for constitutively active chick BMP receptor 1B

   Depositor

   Peter ten Dijke, Lee Niswander

   Article

   Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

   Insert

   BMPR-1B CA (BMPR1B Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   constitutively active mutation (Q203D); also P2A,…

   Promoter

   T3

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pSBbi-FoxO1_1R_10A_3D

   Plasmid

   #106278

   Purpose

   Fluorescent fusion protein for FoxO1

   Depositor

   Laura Heiser

   Article

   Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

   Inserts

   Foxo1a (Foxo1 Mouse)
   NLS-mCherry-NLS-P2A-Puromycin
   

   Use

   Tags

   Clover fluorescent protein

   Expression

   Mammalian

   Mutation

   Deleted amino acids 401-636, S209A, H212R, S215A,…

   Promoter

   EF1a/RPBSA

   Available since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-NRCH-ABE8.17-m+V106W

   Plasmid

   #198551

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-ABE8.17-m+V106W
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENN491_TALEcharm_MmT8x

   Plasmid

   #220836

   Purpose

   TALEcharm Kv2 expression in mammalian cells; targets Prnp

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   TALEcharm
   

   Use

   Lentiviral and TALEN

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EF1a

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENN456_TALEcharm_Kv2_MmT8x

   Plasmid

   #220835

   Purpose

   TALEcharm expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   TALEcharm Kv2
   

   Use

   Lentiviral and TALEN

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EF1a

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-Hsp-Fluc-PGK-Rluc-mCherry

   Plasmid

   #191863

   Purpose

   Heat inducible Fluc reporter with constitutive Rluc

   Depositor

   Yingxiao Wang

   Article

   Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

   Inserts

   Firefly luciferase Fluc
   Renilla luciferase Rluc
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   HSPA7 promoter and PGK promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK427 (OsTIR1(WT) mAID-EGFP-Nluc)

   Plasmid

   #140658

   Purpose

   OsTIR1(WT)-P2A-mAID-EGFP-Nluc

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(WT) mAID-EGFP-Nluc TOL2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-PE

   Plasmid

   #162796

   Purpose

   All-in-one prime editor piggyBac transposon

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUFlip-floxed2A-KalTA4-; HE1A:BFP -0

    Plasmid

    #180010

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

    Plasmid

    #180011

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

    Plasmid

    #180012

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTon1(il11)

    Plasmid

    #233647

    Purpose

    Expression vector of X. laevis il11.L-P2A-AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.

    Depositor

    Takeo Kubo

    Article

    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10

    Inserts

    interleukin-11.L (350-913) (il11.L Frog)
    target sequence of guide RNA #tyr (3905-3927) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    

    Use

    Amphibian expression, tet-on

    Tags

    Expression

    Mutation

    Some synonymous substitutions were introduced in…

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-dCpf1-eBE

    Plasmid

    #107688

    Purpose

    Expresses dLbCpf1-eBE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-eBE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCpf1(D832A/E925A/D1148A)

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pY31

    Plasmid

    #84746

    Purpose

    Expresses huLbCpf1-P2A-puro and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    puromycin resistance gene
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

    Plasmid

    #171615

    Purpose

    Soma-targeted Cal-Light with KA2

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

    Plasmid

    #216763

    Purpose

    PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9-NRCH-APOBEC-nCas9-Ung

    Plasmid

    #198552

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-APOBEC-nCas9-Ung
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pD2529-CAG-b3

    Plasmid

    #189797

    Purpose

    Expression of full length human integrin beta 3

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    integrin b3 (ITGB3 Human)
    

    Use

    Tags

    -P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

    Plasmid

    #140535

    Purpose

    OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    mAID-EGFP-NLS TOL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge