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pFvL118

Plasmid

#64752

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pTW081

Plasmid

#64755

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-RvLEAM

Plasmid

#90035

Purpose

To express GFP-tagged RvLEAM in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

RvLEAM

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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Lmajor_gp63_10_0460_E265A

Plasmid

#171641

Purpose

Expression of catalytically inactive L. major glcyoprotein-63 (E265A) from chromosome 10 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al Front Cell Infect Microbiol. 2019 Aug 7;

Insert

LmjF.10.0460 E265A

Tags

Myc-His

Expression

Mammalian

Mutation

E265A

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEarleyGate103 mmPing20F

Plasmid

#140007

Purpose

mmPing20F activation tag

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20F

Expression

Plant

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.iGABASnFR.F102G

Plasmid

#112167

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Mutation

F102G

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1079A_K1080A

Plasmid

#91861

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) R0179A/K1080A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1080A

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-HT/prsA

Plasmid

#73624

Purpose

Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-rab5c-DN

Plasmid

#109737

Purpose

Multisite gateway vector for 3' tagging with dominant negative rab5c

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5c (rab5c Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8b in pCSDEST2

Plasmid

#140881

Purpose

Expression of zebrafish GFP-tagged Rab8b in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab8b (rab8b Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.R205A

Plasmid

#112178

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.R205A

Expression

Mammalian

Mutation

R205A (no GABA binding)

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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BC-A1-002

Plasmid

#78689

Purpose

A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator

Use

Synthetic Biology

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_E299A

Plasmid

#91855

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) E299A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

E299A

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ1202

Plasmid

#44484

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, peel-1, MCS

Use

Multiple cloning site vector

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pT3TS-Dest_R1-R3

Plasmid

#140878

Purpose

166571: Multisite gateway destination vector for in vitro transcription of RNA using T3. Accepts one pME and one p3E entry clone.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb/CAM

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pYFAC-CH1

Plasmid

#168977

Purpose

AMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacI

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in fil…

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CaaX(tH)

Plasmid

#109487

Purpose

Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Hras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-CaaX(tH)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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JC101: pMVP/Ad/PK-DEST

Plasmid

#121845

Purpose

pMVP destination vector, empty pK fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.iGABASnFR.F102Y.Y137L

Plasmid

#112168

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

AAV

Mutation

F102Y.Y137L

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRNA-U6-miR122 sponge

Plasmid

#35665

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

U6 miR-122sponge

Promoter

U6

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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