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We narrowed to 11,436 results for: 112

Showing: 8551 - 8600 of 11436 results

  1. pRAN357

    Plasmid
    #120898
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein
    Depositor
    David S. Weiss, Craig Ellermeier
    Article
    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.
    Insert
    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120898-plasmid-map-sequence-id-235310

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  5. pWPT-/UAA-mEGFP-IRES-mCherry

    Plasmid
    #49234
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49234-plasmid-map-sequence-id-73657

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  11. pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG

    Plasmid
    #129758
    Purpose
    Dox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.
    Insert
    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    changed Glutamic acid 36 to Alanine and Leucine 3…
    Promoter
    TRE3Gs and Tet inducible promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129758-plasmid-map-sequence-id-257095

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  12. pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49228
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49228-plasmid-map-sequence-id-73645

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  19. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid
    #133347
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_cpaA and sgRNA_blaA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133347-plasmid-map-sequence-id-269540

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  21. pMflPT-o4

    Plasmid
    #101315
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    pac resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101315-plasmid-map-sequence-id-198861

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  22. pMflST-o4

    Plasmid
    #101316
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    aadA1 resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101316-plasmid-map-sequence-id-199230

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  24. pTXB1-xlH2B(1-114)_K114A

    Plasmid
    #118217
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Use
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Mutation
    Lysine 114 to alanine
    Promoter
    T7
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118217-plasmid-map-sequence-id-226718

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  27. pWPT-UCU/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49232
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49232-plasmid-map-sequence-id-73653

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  30. GST-Delta23C11orf83

    Plasmid
    #65849
    Purpose
    bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)
    Depositor
    Amos Bairoch
    Article
    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d
    Insert
    UQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    deletion of the 23 first aa (TM)
    Promoter
    Available since
    July 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    65849-plasmid-map-sequence-id-112004

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  31. pCru5-GGG/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49221
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49221-plasmid-map-sequence-id-73631

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  32. pCru5-UCU/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49223
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49223-plasmid-map-sequence-id-73635

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  33. pCru5-ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49219
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49219-plasmid-map-sequence-id-73627

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  34. cBEST4

    Plasmid
    #234660
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3 and tcp830
    Available since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234660-plasmid-map-sequence-id-472864

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  39. pCAGEN_C74_eMagAF_V5_NES

    Plasmid
    #235636
    Purpose
    Encodes fusion protein of ALIBi C-terminal fragment (residues 74-321), eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence (order reversed)
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    ALIBi C-terminal fragment
    Use
    Tags
    eMagAF-V5-NES
    Expression
    Mammalian
    Mutation
    residues 74-321
    Promoter
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    235636-plasmid-map-sequence-id-467579

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  46. pCAGEN_MyrPalm_NES_V5_eMagAF_C74

    Plasmid
    #235639
    Purpose
    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, MyrPalm for targeting to plasma membrane
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    ALIBi C-terminal fragment
    Use
    Tags
    MyrPalm-NES-V5-eMagAF
    Expression
    Mammalian
    Mutation
    residues 74-321
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235639-plasmid-map-sequence-id-467543

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  47. pCAGEN_V5_eMagAF_C74_cMycNLS

    Plasmid
    #235640
    Purpose
    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, cMyc nuclear localization sequence for targeting to nucleus
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    ALIBi C-terminal fragment
    Use
    Tags
    V5-eMagAF and cMycNLS
    Expression
    Mammalian
    Mutation
    residues 74-321
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235640-plasmid-map-sequence-id-467541

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  49. pCAGEN_NES_V5_eMagAF_C74_Sec61b

    Plasmid
    #235637
    Purpose
    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, Sec61b for targeting ALIBi to endoplasmic reticulum
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    ALIBi C-terminal fragment
    Use
    Tags
    NES-V5-eMagAF and Sec61b
    Expression
    Mammalian
    Mutation
    residues 74-321
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235637-plasmid-map-sequence-id-467535

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Showing: 8551 - 8600 of 11436 results