We narrowed to 38,497 results for: LAT;

1. pmRFP-N1 human cofilin S3E

   Plasmid

   #50858

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA5/FRT/TO-Intron-CterFlag-MePCE

   Plasmid

   #113549

   Purpose

   Mammalian expression plasmid that can be used with the Flp-In T-REx system.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   MePCE (MEPCE Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-MTS-WT-c-Src-FLAG

   Plasmid

   #44652

   Depositor

   Yoshimi Homma

   Article

   Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

   Insert

   v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
   

   Use

   Tags

   FLAG tag and mitochondria-targeting sequence (res…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTET2-lucCP-3MS2

   Plasmid

   #198351

   Purpose

   Part of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTR

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   Firefly Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTET2

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJZC34

   Plasmid

   #62331

   Purpose

   sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 2x MS2(wt+f6) binding module
   MCP-VP64
   

   Use

   Lentiviral

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

   Promoter

   CMV and U6

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.2/NS5-YFP-NLS-mut

   Plasmid

   #120832

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   ZIKV NS5-eYFP "NLS-mut"
   

   Use

   Tags

   eYFP-V5

   Expression

   Mammalian

   Mutation

   R372A/Q373A/K387A/H388A/K389A

   Promoter

   CMV

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR KIF17 (1-738)

   Plasmid

   #60406

   Purpose

   Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   KIF17 (KIF17 Human)
   

   Use

   Lentiviral

   Tags

   GB1, Strep-Tag, and sfGFP

   Expression

   Mammalian

   Mutation

   Truncated version amino acids 1-738

   Promoter

   SFFV

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVX neo PFKP-GFP K281R

   Plasmid

   #138288

   Purpose

   Mammalian Expression, Lentiviral. Expression of human phosphofructokinase platelet isoform PFKP-GFP fusion protein with K281R mutation

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   PFKP (PFKP Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K281R

   Promoter

   CMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSF4 CMV 5TOP intron renilla TRICK CTE polyA

   Plasmid

   #64544

   Purpose

   5′ TOP TRICK reporter mRNA

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   12x PP7 stem-loops
   

   Use

   Tags

   24xMS2 stem loops, 5' TOP (5′ terminal oligo…

   Expression

   Mammalian

   Mutation

   Promoter

   Tetracycline-inducible promoter and 5′ UTR of hum…

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hnRNPA2_LC_nocharge

    Plasmid

    #139118

    Purpose

    express hnRNPA2 LC with no charged residues

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all charged residues are changed to S/Q except fo…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-FLAG-IRES-blasticidin-Sox2 WT

    Plasmid

    #182046

    Purpose

    expression of Sox2

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPPC011

    Plasmid

    #171143

    Purpose

    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Sp.pCas9, BBa_J23107

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TMED10-bio-His

    Plasmid

    #51852

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED10 (TMED10 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJZC78

    Plasmid

    #62339

    Purpose

    sgRNA + 1x COM with COM-KRAB effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1x COM binding module
    COM-KRAB
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

    Promoter

    CMV and U6

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PPP1CA

    Plasmid

    #155843

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PPP1CA (PPP1CA Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-4EBP1 F113A

    Plasmid

    #81122

    Purpose

    expresses a constitutively active mutant of 4E-BP1 under the CAG promoter

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    4EBP1 F113A (Eif4ebp1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F113A mutation

    Promoter

    CAG

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIRESpuroN3xFLAG-UFSP2 C302A

    Plasmid

    #204554

    Purpose

    Expresses 3xFLAG-tagged UFSP2 C302A in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    UFSP2 (UFSP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C302A

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJZC33

    Plasmid

    #62330

    Purpose

    sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2x MS2 binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMIR-21 Reporter

    Plasmid

    #71870

    Purpose

    Mammalian expression vector for the analysis of miR-21 activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-21
    

    Use

    Tags

    firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO-1 shRev-Erbalpha

    Plasmid

    #22747

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    shRev-Erbalpha (Nr1d1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only