We narrowed to 34,848 results for: CaS;

1. pX459_gRNA pAS dual_hspCas9

   Plasmid

   #193312

   Purpose

   Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXT128_TwinStrep-SUMO-ShCas12k

   Plasmid

   #135524

   Purpose

   Protein expression construct for ShCast12k

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShCas12k
   

   Use

   Tags

   TwinStrep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459V2.0-eSpCas9(1.1)

   Plasmid

   #108292

   Purpose

   pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. dxCas(3.7)-VPR

   Plasmid

   #108383

   Purpose

   Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   dxCas(3.7)-VPR
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. dCas9-10xSunTag(22aa)

   Plasmid

   #215745

   Purpose

   Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   dCas9-10xSunTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta-actin promoter

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_TOMM20 sgRNA / hSpCas9

   Plasmid

   #172836

   Purpose

   Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b-DiCas7-11-His

   Plasmid

   #234482

   Purpose

   Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   DiCas7-11
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330a dCas9-LSD1

   Plasmid

   #92362

   Purpose

   Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9-LSD1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. TccCas13a Twin SUMO

   Plasmid

   #199754

   Purpose

   Expresses codon optimized thermostabile TccCas13a in E.coli

   Depositor

   Magdy Mahfouz

   Article

   Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

   Insert

   TccCas13a
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   LacI

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-Myc-Caspase-5

    Plasmid

    #214305

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP5 (CASP5 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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