Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 6,273 results for: nls

Clear keywords
Showing: 3441 - 3460 of 6273 results

  4. hnRNPA1_allW_D262V-FLAG-IRES-eGFP

    Plasmid
    #234621
    Purpose
    Mammalian expression of full-length hnRNPA1 D262V with all aromatic amino acids in the C-terminal LCD mutated to W keeping the hexapeptide fibril core (SYNDFG) and the PY-NLS intact.
    Depositor
    Tanja Mittag
    Article
    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2
    Insert
    hnRNPA1 allW D262V (HNRNPA1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Famillial ALS mutation D262V, all aromatic amino …
    Promoter
    CMV
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    234621-plasmid-map-sequence-id-464954

    Enlarge

  7. pCUU

    Plasmid
    #159747
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159747-plasmid-map-sequence-id-313815

    Enlarge

  8. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid
    #196720
    Purpose
    Tet-inducible expression of Cas9-T2A-TagBFP
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Promoter
    TRE, PGK
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196720-plasmid-map-sequence-id-389756

    Enlarge

  9. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

    Enlarge

  10. pCMV-ABE-P48R

    Plasmid
    #161816
    Purpose
    Expresses ABE-P48R in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    161816-plasmid-map-sequence-id-320046

    Enlarge

  11. HaloTag-LacI

    Plasmid
    #232636
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag
    Depositor
    Karsten Rippe
    Insert
    HaloTag-LacI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232636-plasmid-map-sequence-id-462868

    Enlarge

  12. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid
    #196722
    Purpose
    Tet-inducible expression of Cas9-T2A-miRFP670nano
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Promoter
    TRE, PGK
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196722-plasmid-map-sequence-id-389804

    Enlarge

  13. pHygRB12

    Plasmid
    #244899
    Purpose
    hygR selection nls-Scarlet marked rapid RMCE integration vector with an attB site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244899-plasmid-map-sequence-id-485995

    Enlarge

  14. pHygGB16

    Plasmid
    #244896
    Purpose
    hygR selection nls-GFP marked rapid RMCE integration vector with an attB site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244896-plasmid-map-sequence-id-485985

    Enlarge

  15. pHygGP16

    Plasmid
    #244903
    Purpose
    hygR selection nls-GFP marked rapid RMCE integration vector with an attP site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244903-plasmid-map-sequence-id-485493

    Enlarge

  16. pHygRP12

    Plasmid
    #244905
    Purpose
    hygR selection nls-Scarlet marked rapid RMCE integration vector with an attP site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244905-plasmid-map-sequence-id-485485

    Enlarge

  18. pb-CAGGS-dCas9-blasto

    Plasmid
    #218555
    Purpose
    Encodes CAG promoter-driven dCas9 with a C-terminal bipartite SV40 NLS, along with blasticidin resistance
    Depositor
    Alejandro Chavez
    Insert
    dCas9
    Use
    CRISPR; Piggybac transposition
    Tags
    2xSV40NLS
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    218555-plasmid-map-sequence-id-431930

    Enlarge

  20. PHGDH

    Plasmid
    #38841
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHGDH (PHGDH Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 3441 - 3460 of 6273 results