We narrowed to 6,087 results for: nls

1. pVITRO1-SS-257

   Plasmid

   #68774

   Purpose

   C Trans V2

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Insert

   C-terminal Sensor 1 V2
   

   Use

   Tags

   NLS and flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNXRVc-HACre

   Plasmid

   #78400

   Purpose

   constitutive Cre expression vector

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Inserts

   3xHA-nls-Cre
   natMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX403 Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

   Plasmid

   #68337

   Purpose

   Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

   Depositor

   Feng Zhang

   Insert

   St3Cas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPS1372

   Plasmid

   #1353

   Depositor

   Pamela Silver

   Article

   Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

   Insert

   pADH-NLS-NES-GFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pBW121-SS-273

   Plasmid

   #68763

   Purpose

   ZF9-TF-V2 with N-FLAG and C-HIS

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Insert

   ZF9-TF-V2
   

   Use

   Tags

   HIS, NLS, and flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNXRVbt-HACre

   Plasmid

   #78402

   Purpose

   constitutive Cre expression vector

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Inserts

   3xHA-nls-Cre
   HyTkAX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2

   Plasmid

   #218557

   Purpose

   Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

   Depositor

   Alejandro Chavez

   Insert

   MCP-HSF1-VIRF2
   

   Use

   Lentiviral

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   Available since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. (829) SB Tet-ON dCasphi-[myc-ZIM3-HA] U6-gStop

   Plasmid

   #195622

   Purpose

   Sleeping Beauty Tet-ON plasmid for induced expression of dCasphi-ZIM3 CRISPRi construct

   Depositor

   Tonia Rex

   Insert

   dCasphi
   

   Use

   Tags

   C-Myc (NLS), HA tag, and ZIM3

   Expression

   Mammalian

   Mutation

   P749A and P753A

   Promoter

   Tight TRE promoter; Tet-ON (doxycycline)

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. (942) pAAV EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

   Plasmid

   #195624

   Purpose

   pAAV EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct

   Depositor

   Tonia Rex

   Insert

   dCasphi
   

   Use

   AAV

   Tags

   C-Myc (NLS), HA tag, and ZIM3

   Expression

   Mammalian

   Mutation

   P749A and P753A

   Promoter

   EF1a (Mlu1/EcoR1)

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. iC30 PB-Zim3-dCas9-P2A-BFP_hygro

    Plasmid

    #236743

    Purpose

    Constitutive expression of Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    CAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
    EF1a-hygro-WPRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. GLuc VLP

    Plasmid

    #182213

    Purpose

    YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Gaussia luciferase tagged with VP2C (GAL10 promoter).

    Depositor

    Claudia Vickers

    Inserts

    VP2C-GLuc
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-BLAST PHGDH

    Plasmid

    #154917

    Purpose

    Expresses human PHGDH

    Depositor

    Richard Possemato

    Article

    Richard et al Richard Possemato Lab plasmids

    Insert

    PHGDH (PHGDH Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNXRVct-HACre

    Plasmid

    #78403

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    TKnatAX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVITRO1-SS-255

    Plasmid

    #68773

    Purpose

    C Trans V1

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    C-Terminal Sensor 1 V1
    

    Use

    Tags

    NLS and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVITRO1-SS-259

    Plasmid

    #68775

    Purpose

    C Trans V3

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    C-Terminal Sensor 1 V3
    

    Use

    Tags

    NLS and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT3TS-nCas9n

    Plasmid

    #46757

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T3

    Available since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQP-T2A

    Plasmid

    #102583

    Purpose

    Light-controlled activation of expression

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

    Insert

    BphP1-VP16-T2A-NLS-GAL4-QPAS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBFv-nosP-Cas9

    Plasmid

    #138402

    Purpose

    Expresses Cas9 in the germline of Drosophila

    Depositor

    Shu Kondo

    Article

    Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

    Insert

    Cas9-NLS
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pG14-MS2-GFP

    Plasmid

    #27117

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    MS2-binding protein
    

    Use

    Tags

    GFP, HA, and NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRP2370

    Plasmid

    #127576

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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