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We narrowed to 6,447 results for: nls

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Showing: 3441 - 3460 of 6447 results

  1. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  2. pCAGEN_V5_eMagAF_C74_cMycNLS

    Plasmid
    #235640
    Purpose
    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, cMyc nuclear localization sequence for targeting to nucleus
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    ALIBi C-terminal fragment
    Tags
    V5-eMagAF and cMycNLS
    Expression
    Mammalian
    Mutation
    residues 74-321
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235640-plasmid-map-sequence-id-467541

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  6. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

    Plasmid
    #84243
    Purpose
    Expresses ABA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    ABI-tagBFP-Sa dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available Since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84243-plasmid-map-sequence-id-160884

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  13. mKO2-PCNA-19-SV40NLS-4

    Plasmid
    #57890
    Purpose
    Localization: Nucleus, Excitation: 551, Emission: 565
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mKO2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    57890-plasmid-map-sequence-id-233994

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Showing: 3441 - 3460 of 6447 results