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We narrowed to 14,168 results for: eng

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Showing: 3461 - 3480 of 14168 results

  6. pcU6_3 MS2 sgRNA

    Plasmid
    #92394
    Purpose
    Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    chick U6.3
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    92394-plasmid-map-sequence-id-191866

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  8. pSH-Csy4-T2A-SaRFN

    Plasmid
    #85755
    Purpose
    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-SadCas9
    Use
    CRISPR
    Tags
    3xHA tag, Csy4, and FokI fusion via 36 amino acid…
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    CAG
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85755-plasmid-map-sequence-id-170274

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  12. AAV-Syn-NS1

    Plasmid
    #175276
    Purpose
    AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS
    Depositor
    Wei Xu
    Publication
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    NLS-dTomato (P2A cleavage)
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    175276-plasmid-map-sequence-id-349086

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  13. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162668-plasmid-map-sequence-id-321523

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Showing: 3461 - 3480 of 14168 results