We narrowed to 4,990 results for: mcs

1. pJL1-mTagBFP2

   Plasmid

   #102638

   Purpose

   In vitro expression of mTagBFP2 from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   mTagBFP2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT282 Lenti pEF-dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast

   Plasmid

   #187337

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-puro/Flag PD-L1 N192Q/N200Q

   Plasmid

   #121453

   Purpose

   expression of human PD-L1 mutant in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N192Q/N219Q

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-puro/Flag PD-L1 N219Q

   Plasmid

   #121452

   Purpose

   expression of huamn PD-L1 mutant in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N219Q

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL1-mOrange

   Plasmid

   #102632

   Purpose

   In vitro expression of mOrange from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   mOrange
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. JDW 742 (pDONR221-EGFP-KRAS4B-G12V)

   Plasmid

   #156403

   Purpose

   Gateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codon

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4B-G12V (KRAS Human)
   

   Use

   Gateway cloning

   Tags

   EGFP

   Mutation

   mutant KRAS4B-G12V

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-puro/Flag PD-L1 N200Q

   Plasmid

   #121451

   Purpose

   expression of human PD-L1 mutant in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N200Q

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-GFAP-eGFP-shAqp4-4

   Plasmid

   #223225

   Purpose

   mir30 based shRNA strategy, shRNA targeting mouse Aqp4

   Depositor

   Li-Huei Tsai

   Article

   Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

   Insert

   shAqp4 (Aqp4 Mouse)
   

   Use

   AAV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-GFAP-eGFP-shAqp4-3

   Plasmid

   #223224

   Purpose

   mir30 based shRNA strategy, shRNA targeting mouse Aqp4

   Depositor

   Li-Huei Tsai

   Article

   Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

   Insert

   shAqp4 (Aqp4 Mouse)
   

   Use

   AAV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-GFAP-eGFP-shAqp4-1

    Plasmid

    #223223

    Purpose

    mir30 based shRNA strategy, shRNA targeting mouse Aqp4

    Depositor

    Li-Huei Tsai

    Article

    Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

    Insert

    shAqp4 (Aqp4 Mouse)
    

    Use

    AAV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIG-832_NY-ESO-1_TCR_FAS/4-1BB

    Plasmid

    #207495

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/4-1BB, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIG-835_NY-ESO-1_TCR_FAS/MyD88

    Plasmid

    #207498

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/MyD88, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJL1-YPet

    Plasmid

    #102633

    Purpose

    In vitro expression of YPet from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    YPet
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR223-CMV-Cre-U6-sgX-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

    Plasmid

    #209060

    Purpose

    Entry cloning vector to insert an sgRNA of interest (using Esp3i digestion) into a vector that already contains sgRNAs against mouse Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

    Depositor

    Matthew Oser

    Article

    Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

    Insert

    sgRNAs targeting Rb1, Trp53, Rbl2 and Cre recombinase
    

    Use

    Gateway vector to be used for lr reaction

    Promoter

    U6

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #187324

    Purpose

    Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
    

    Use

    CRISPR and TALEN

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL1-mTFP1

    Plasmid

    #102635

    Purpose

    In vitro expression of mTFP1 from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    mTFP1
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)

    Plasmid

    #156410

    Purpose

    pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A-G12V

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12V (KRAS Human)
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    mutant KRAS4A-G12V

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)

    Plasmid

    #156409

    Purpose

    pCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12D

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    mutant KRAS4A-G12D

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIG-834_NY-ESO-1_TCR_FAS/IL4R

    Plasmid

    #207497

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/IL4R, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. JDW 743 (pDONR221-EYFP-hKRAS4B-WT)

    Plasmid

    #156402

    Purpose

    Gateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4B-WT (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    EYFP

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge