We narrowed to 9,679 results for: pho

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Flag-hPKL S113D

Plasmid

#107161

Purpose

For mammalian expression of Flag-hPKL S113D

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL S113D (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Mutation

Serine 113 mutated to aspartate

Promoter

CMV

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-hPKL S113A

Plasmid

#107160

Purpose

For mammalian expression of Flag-hPKL S113A

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL S113A (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Mutation

Serine 113 mutated to alanine

Promoter

CMV

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221 - myc ULK1 4SA

Plasmid

#27625

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 4SA (Myc Mouse)

Use

Gateway donr vector

Tags

myc

Mutation

S467A, S555A, T574A, S637A. Initial M from ULK1 r…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-ARAP3(C504A)

Plasmid

#39485

Depositor

Sonja Vermeren

Article

Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

Insert

ARAP3(C504A) (ARAP3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C504A = Arf GAP domain mutation

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pDEST26 Ago2 5A

Plasmid

#60651

Purpose

Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

mutated five serines to alanines

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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PiggyBac CAG eGFP-PolQ-FLAG P2A-BLAST

Plasmid

#249811

Purpose

Expresses the PolQ (polymerase theta) protein fused with N-terminal eGFP and C-terminal FLAG tag

Depositor

Raphael Ceccaldi

Article

Gelot et al Nature. 2023 Sep;621(7978):415-422. doi:

Insert

Polymerase Theta (POLQ Human)

Tags

FLAG and eGFP

Expression

Mammalian

Promoter

CAG

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR2b-ITGB1(YYFF)-mRuby2

Plasmid

#215447

Purpose

Gateway entry vector with integrin beta1 NPxY mutant (YYFF) tagged with mRuby2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Tags

mRuby2

Mutation

Mutations in the NPxY sites Y783F, Y795F; Silent …

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB.DEST-ITGB1(YYFF)-mRuby2

Plasmid

#215449

Purpose

Piggybac expression vector with integrin beta1 NPxY mutant (YYFF) tagged with mRuby2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Transposon-based stable expression

Tags

mRuby2

Expression

Mammalian

Mutation

Mutations in the NPxY sites Y783F, Y795F; Silent …

Promoter

CAG

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBIG1e-CDK12deltaNTD /Cyclin K

Plasmid

#231741

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK12 (CDK12 Human)
CCNK (CCNK Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1490)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1e-CDK12deltaCTD/Cyclin K

Plasmid

#231742

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK12 (CDK12 Human)
CCNK (CCNK Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(1-1082)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1e-CDK12-KiD/Cyclin K

Plasmid

#231743

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK12 (CDK12 Human)
CCNK (CCNK Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1082)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRetroQ-GTSE1-acGFP-S91, 262, 454, 724A

Plasmid

#224741

Purpose

Retroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein with S91, 262, 454, 724A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Use

Retroviral

Tags

acGFP

Mutation

Serine 91, 262, 454, 724 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroQ-GTSE1-acGFP-S91, 262, 454, 724D

Plasmid

#224742

Purpose

Retroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein with S91, 262, 454, 724D mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Use

Retroviral

Tags

acGFP

Mutation

Serine 91, 262, 454, 724 Aspartate; Valine 53 Iso…

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-S170D

Plasmid

#213592

Purpose

Doxycycline inducible expression of myc-tagged ERK1-S170D cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

S170D

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-R84S/D338N

Plasmid

#213594

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/D338N cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, D338N

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBOBI N-HA AXIN1 delta PHGDH (delta 321-340)

Plasmid

#213005

Purpose

expresses mouse AXIN1

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

AXIN1 (Axin1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBOBI N-FLAG HIPK2

Plasmid

#213004

Purpose

expresses mouse HIPK2

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

HIPK2 (Hipk2 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBOBI C-FLAG PHGDH T57A

Plasmid

#212996

Purpose

expresses human PHGDH

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

PHGDH (PHGDH Human)

Tags

FLAG

Expression

Mammalian

Mutation

T57A

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBOBI TP53 S46A

Plasmid

#213001

Purpose

expresses human P53

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

P53 (TP53 Human)

Expression

Mammalian

Mutation

S46A

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-PSK2 (1-416) (K57A)

Plasmid

#197124

Purpose

Expression of kinase-deficient Myc-tagged PSK2 (aa1-416, K57A) / TAOK1 (aa1-416, K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Tavares et al J Biol Chem. 2013 May 24;288(21):15418-2

Insert

TAOK1 (aa1-416, K57A) (TAOK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A and deleted C-terminus

Promoter

SV40

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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