We narrowed to 6,905 results for: crispr cas9 plasmids

1. MSP2440

   Plasmid

   #72250

   Purpose

   Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-dSa-VP64-p65(100-261)-RTA(125-190)

   Plasmid

   #99679

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VP64-p65(101-261)-RTA(125-190)

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMLS287

   Plasmid

   #73730

   Purpose

   SapTrap flexible linker C-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Flexible Linker
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMLS288

   Plasmid

   #73735

   Purpose

   SapTrap flexible linker N-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Flexible Linker
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMLS279

   Plasmid

   #73729

   Purpose

   SapTrap FLP-on C-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   C-terminal FLP-on
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMLS382

   Plasmid

   #73731

   Purpose

   SapTrap Flagtag-TEV C-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   FLAGtag-TEV
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMLS282

   Plasmid

   #73733

   Purpose

   SapTrap FLP-on N-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   N-terminal FLP-on
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMLS383

   Plasmid

   #73736

   Purpose

   SapTrap TEV-Flagtag N-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   TEV-FLAGtag
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonor_mU6

   Plasmid

   #69350

   Purpose

   sgRNA scaffold and mouse U6 promoter

   Depositor

   Andrea Ventura

   Article

   Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

   Insert

   mU6 promoter and sgRNA scaffold flanked by BbsI sites
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   mU6 promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDonor_hU6

    Plasmid

    #69312

    Purpose

    sgRNA scaffold and human U6 promoter

    Depositor

    Andrea Ventura

    Article

    Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

    Insert

    hU6 promoter and sgRNA scaffold flanked by BbsI sites
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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