We narrowed to 11,613 results for: SHA

1. pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI

   Plasmid

   #195502

   Purpose

   Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA (SOX17 Human)
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX2T-TEV CHMP1B 186-199 L188A/L192A

   Plasmid

   #108288

   Purpose

   Expresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed L 188 to A, changed L 192 to A

   Promoter

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX2T-TEV CHMP1B 186-199 L192A/L195A

   Plasmid

   #108290

   Purpose

   Expresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed L 188 to A, changed L 192 to A

   Promoter

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 18065-M01-412

   Plasmid

   #225664

   Purpose

   Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

   Depositor

   Dominic Esposito

   Article

   Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

   Inserts

   dsCopGFP
   delCD19 (CD19 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   C-terminally truncated (aa 1-333 only)

   Promoter

   SORE6-mCMVp and WPRE-SV40p

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 18065-M02-412

   Plasmid

   #225665

   Purpose

   Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

   Depositor

   Dominic Esposito

   Article

   Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

   Inserts

   dsmCherry
   delCD19 (CD19 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   C-terminally truncated (aa 1-333 only)

   Promoter

   SORE6-mCMVp and WPRE-SV40p

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR8/GW_TOPO_Cdk13_Nterm_FlagHa

   Plasmid

   #127343

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Cyclin Dependent Kinase 13 (Cdk13 Mouse)
   

   Use

   Entry vector with transgene for gateway cloning

   Tags

   Flag- HA- Tandem Epitopes

   Expression

   Mutation

   Base pairs 1-1554 of transgene are codon optimize…

   Promoter

   None

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-Neo-TetO-Cdk13_Untagged

   Plasmid

   #127344

   Purpose

   Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Cyclin Dependent Kinase 13 (Cdk13 Mouse)
   

   Use

   Piggybac transposase vector

   Tags

   None

   Expression

   Mammalian

   Mutation

   Base pairs 1-1554 of transgene are codon optimize…

   Promoter

   TetO Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-Neo-TetO-Cdk13_Nterm_FlagHa

   Plasmid

   #127345

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Cyclin Dependent Kinase 13 (Cdk13 Mouse)
   

   Use

   Piggybac transposase vector

   Tags

   Flag- HA- Tandem Epitopes

   Expression

   Mammalian

   Mutation

   Base pairs 1-1554 of transgene are codon optimize…

   Promoter

   TetO Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMIR-Report-RASA1-3'UTR (mut - miR-206 site)

   Plasmid

   #62576

   Purpose

   Translational Luciferase Reporter containing the mutated 3'UTR of RASA1. The miR-206 binding site was mutated.

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   RASA1 (RASA1 Human)
   

   Use

   Luciferase

   Tags

   None

   Expression

   Mutation

   miR-206 binding site mutated (ACATTCCA --> AAC…

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pSH1.6EGFP

    Plasmid

    #42323

    Depositor

    Amir Sharon, Holger Deising

    Insert

    EGFP
    

    Use

    Filamentous ascomycetes egfp expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Zeo-otmyd88

    Plasmid

    #217485

    Purpose

    Expression of Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon

    Depositor

    Bertrand Collet

    Insert

    BFP-P2A-otPKRFL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 myc-PITPbeta

    Plasmid

    #58278

    Purpose

    mammalian expression of rat PITP beta

    Depositor

    Shamshad Cockcroft

    Insert

    PITP beta (Pitpnb Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1 myc-PITPalpha

    Plasmid

    #58277

    Purpose

    mammalian expression of human PITP alpha

    Depositor

    Shamshad Cockcroft

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-MK1

    Plasmid

    #29638

    Depositor

    Bruce Tempel

    Insert

    Mk1 (Kcna1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 5, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pUK21-deltaBB

    Plasmid

    #51658

    Purpose

    Bacterial cloning vector based on pUK21 (Kan)

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC21-deltaBB

    Plasmid

    #51657

    Purpose

    Bacterial cloning vector based on pUC21 (Amp)

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGs-RFP

    Plasmid

    #127347

    Purpose

    Expresses RFP constitutively via the CAGGS promoter in mammalian cells

    Depositor

    Phil Sharp

    Insert

    Red Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS Promoter

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGs-BFP

    Plasmid

    #127348

    Purpose

    Expresses BFP constitutively via the CAGGS promoter in mammalian cells

    Depositor

    Phil Sharp

    Insert

    Blue Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGs-Venus

    Plasmid

    #127346

    Purpose

    Expresses Venus constitutively via the CAGGS promoter in mammalian cells

    Depositor

    Phil Sharp

    Insert

    Venus Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p1153 BPV-1 E2-TR

    Plasmid

    #10847

    Depositor

    Peter Howley

    Article

    Lambert et al Cell. 1987 Jul 3. 50(1):69-78.

    Insert

    BPV-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge