We narrowed to 22,705 results for: his
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Plasmid#200792PurposeMammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
TagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutationΔ954-1027PromoterCMV & T7Available SinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG
Plasmid#200798PurposeMammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
TagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutation411-680PromoterCMV & T7Available SinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)
Plasmid#227611PurposeInducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastorisDepositorTagsMBP, 10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-88 onlyPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)
Plasmid#227610PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastorisDepositorTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-400, 706-757 [TM and HB2 deleted]PromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268
Plasmid#227089PurposeBacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)DepositorInserttom1p-HECT (TOM1 Budding Yeast)
TagsHIS-MBP-TEV_cleavage_siteExpressionBacterialPromoterT7Available SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR
Plasmid#169135PurposeE. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertCoh7
Tags3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…ExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EV-D68 2A protease; 6xHis-SUMO, low expression levels
Plasmid#204802PurposeBacterial expresison of Enterovirus D68 2A proteaseDepositorInsertD68 2A protease
ExpressionBacterialAvailable SinceAug. 29, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac HTB His-TEV-rDPP9(S729A)-FLAG
Plasmid#217329PurposePurification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cellsDepositorInsertDPP9
TagsFLAG and His-TEVExpressionInsectMutationS729APromoterpolyhedrinAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1(N61K)-FLAG
Plasmid#217325PurposePurification of Brown Norway rat NLRP1 (N61K mutation) fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
TagsFLAG and His-TEVExpressionInsectMutationN61KPromoterpolyhedrinAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG
Plasmid#217324PurposePurification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
TagsFLAG and His-TEVExpressionInsectMutationS969APromoterpolyhedrinAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-LEW-rNLRP1-FLAG
Plasmid#217320PurposePurification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
TagsFLAG and His-TEVExpressionInsectPromoterpolyhedrinAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1202-pOCC175_pOEM1-N-HIS-MBP-PS-mGFP-TEV-HNRNPA3_opt
Plasmid#221890PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertHNRNPA3 (HNRNPA3 Human)
Tags6xHis-MBP-PreScission-mGFP-TEVExpressionInsectMutationwtPromoterPH promoterAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS58_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)] - I3-01
Plasmid#211890PurposeBacterial expression of CENP-T[1-242/3D]-I3-01DepositorInsertCENP-T[1-242/3D]-I3-01 (CENPT Synthetic, Human)
ExpressionBacterialMutationT11D, T27D, T85DAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS275_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)]
Plasmid#211891PurposeBacterial expression of CENP-T[1-242/3D]DepositorAvailable SinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP
Plasmid#212795PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeastDepositorInsertHis6-CUbo(K48R/K63R)-GFP
UseIntegrative vectorExpressionYeastMutationCUb(K48R/K63R)PromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF_1-640-del331-370-V5His6_AH
Plasmid#148906PurposeInsect Expression of DmGIGYF_1-640-del331-370DepositorInsertDmGIGYF_1-640-del331-370 (Gyf Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-DmRoquin_702-819-del790-812-GB1His6_AE
Plasmid#148623PurposeBacterial Expression of DmRoquin_702-819-del790-812DepositorInsertDmRoquin_702-819-del790-812 (roq Fly)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-DmRoquin_702-819-del725-755-GB1His6_AE
Plasmid#148622PurposeBacterial Expression of DmRoquin_702-819-del725-755DepositorInsertDmRoquin_702-819-del725-755 (roq Fly)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-del28-43-V5His6_Z
Plasmid#148157PurposeInsect Expression of Dm4E-T_1-511-del28-43DepositorInsertDm4E-T_1-511-del28-43 (4E-T Fly)
ExpressionInsectMutationtwo non silent mutations L127S, annotated as EST,…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-FWKtoAAA-V5His6_Z
Plasmid#148158PurposeInsect Expression of Dm4E-T_1-511-FWKtoAAADepositorInsertDm4E-T_1-511-FWKtoAAA (4E-T Fly)
ExpressionInsectMutationtwo non silent mutations L127S, annotated as EST,…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-Y10AL15A-V5His6_Z
Plasmid#148161PurposeInsect Expression of Dm4E-T_1-511-Y10AL15ADepositorInsertDm4E-T_1-511-Y10AL15A (4E-T Fly)
ExpressionInsectMutationtwo non silent mutations L127S, annotated as EST,…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-YLLLtoAAAA-V5His6_Z
Plasmid#148162PurposeInsect Expression of Dm4E-T_1-511-YLLLtoAAAADepositorInsertDm4E-T_1-511-YLLLtoAAAA (4E-T Fly)
ExpressionInsectMutationtwo non silent mutations L127S, annotated as EST,…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T_1-510-Y10AL15A-V5His6_T
Plasmid#147600PurposeInsect Expression of Dm4E-T_1-510-Y10AL15ADepositorInsertDm4E-T_1-510-Y10AL15A (4E-T Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot4_275-575-del377-402-GB1His6_AG
Plasmid#148785PurposeE. coli of HsNot4_275-575-del307-402DepositorInsertHsNot4_275-575-del307-402 (CNOT4 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot4_275-575-del377-424-GB1His6_AG
Plasmid#148786PurposeBacterial Expression of HsNot4_275-575-del377-424DepositorInsertHsNot4_275-575-del377-424 (CNOT4 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot4_275-575-del400-428-GB1His6_AG
Plasmid#148787PurposeE. coli of HsNot4_275-575-del400-428DepositorInsertHsNot4_275-575-del400-428 (CNOT4 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-F152E-V5His6_AC
Plasmid#148467PurposeInsect Expression of DmNanos_116-163-F152EDepositorInsertDmNanos_116-163-F152E (nos Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-L127DF130D-V5His6_AC
Plasmid#148468PurposeInsect Expression of DmNanos_116-163-L127DF130DDepositorInsertDmNanos_116-163-L127DF130D (nos Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCup_1-417-4Ala-mut-V5His6_M
Plasmid#146897PurposeInsect Expression of DmCup_1-417-4Ala-mutDepositorInsertDmCup_1-417-4Ala-mut (cup Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del183-258-siRNAres-V5His6_L
Plasmid#146862PurposeInsect Expression of DmPABPC1_del183-258-siRNAresDepositorInsertDmPABPC1_del183-258-siRNAres (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and seven silent mu…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only