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pPL5618_pUG_MtxR

Plasmid

#60929

Purpose

PCR template vector for generating methotrexate resistance cassette.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Dihydrofolate reductase (DHFR Human)

Use

Loxp flanked deletion cassette template

Mutation

L22F F31S

Promoter

TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP (F64L, S65T, V163A)-TRP1

Plasmid

#53184

Purpose

chromosomal tagging with stable GFP

Depositor

John Pringle

Article

Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP (F64L, S65T, V163A)

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
pMP71-mGFP-LLO-mPMEL

Plasmid

#174608

Purpose

expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the murine hPMEL epitope

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

membrane GFP fused to LLO and mouse PMEL antigen

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Axin2 probe

Plasmid

#21277

Depositor

Frank Costantini

Article

Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

Insert

Axin2 (Axin2 Mouse)

Use

For riboprobe synthesis

Mutation

cDNA bp 1774 to 2787

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
pGL3-B-pGAS5

Plasmid

#46371

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

Insert

GAS5 (GAS5 Human)

Use

Luciferase

Tags

luciferase

Mutation

promoter

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pZA26

Plasmid

#158504

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc (ZAR1 Mustard Weed)

Tags

4xMyc

Expression

Plant

Mutation

D489V

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMBA334

Plasmid

#214746

Purpose

High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Klf4

Plasmid

#19764

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG

Plasmid

#127300

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pQmod2E-GG

Plasmid

#191345

Purpose

Clostridium expression vector (pBP1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

Plasmid

#129706

Purpose

Express cre-dependent KASH-EGFP and Gmeb1 shRNA

Depositor

Yi Zhang

Article

Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

Insert

KASH-EGFP

Use

AAV

Tags

EGFP

Expression

Mammalian

Promoter

U6

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMB3-B-TelR

Plasmid

#204992

Purpose

Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GUG-nLuc-3XFLAG

Plasmid

#127301

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SauriCas9-Puro

Plasmid

#135965

Purpose

Expresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/UUG-nLuc-3XFLAG

Plasmid

#127302

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMBA333

Plasmid

#214745

Purpose

Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pHis-zfEpoa

Plasmid

#65611

Purpose

Express soluble His-tagged zebrafish erythropoietin in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2009 Jul 9;114(2):279-89. doi: 10

Insert

erythropoietin (epoa Zebrafish)

Tags

(His)6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pQmod3E-GG

Plasmid

#191348

Purpose

Clostridium expression vector (pCB102 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pQmod4E-GG

Plasmid

#191351

Purpose

Clostridium expression vector (pCD6 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG SMG1 T3550A/S3556A

Plasmid

#199574

Purpose

Expresses FLAG-tagged mutated SMG1

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

T3550A and S3556A

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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