159,557 results

1. Flag-SIRT1

   Plasmid

   #1791

   Purpose

   Mammalian expression of flag-tagged SIRT1

   Depositor

   Michael Greenberg

   Article

   Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

   Insert

   SIRT1 (SIRT1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. hCas9

   Plasmid

   #41815

   Purpose

   Expresses human codon optimized Cas9 nuclease for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUGW-PercevalHR

   Plasmid

   #49083

   Purpose

   Lentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. rEstus

   Plasmid

   #209767

   Purpose

   To express a bright and sensitive genetically encoded voltage indicator in mammalian cells.

   Depositor

   Stefan Heinemann

   Article

   Rühl et al bioRxiv 2023.

   Insert

   rEstus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. XRE-H2B-eGFP

   Plasmid

   #182294

   Purpose

   Fluorescent reporter for AhR activity

   Depositor

   Severine Degrelle, Thierry Fournier

   Article

   Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

   Insert

   Xenobiotic Response Element (CYP1A1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   XRE

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmGFP-ADAR1-p150

   Plasmid

   #117927

   Purpose

   ADAR1-p150 expression plasmid.

   Depositor

   Kumiko Ui-Tei

   Article

   Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

   Insert

   ADAR1-p150 (ADAR Human)
   

   Use

   Tags

   myc-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-SFFV-dCas9-BFP-KRAB

   Plasmid

   #46911

   Purpose

   Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-BFP-KRAB fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2xNLS, BFP, HA, and KRAB domain

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-Streptavidin-Dead

   Plasmid

   #20859

   Purpose

   Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

   Depositor

   Alice Ting

   Article

   Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

   Insert

   Streptavidin-Dead
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Triple mutant of wt Streptavidin N23A,S27D,S45A

   Promoter

   Available since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-EGFR-V5/HIS

   Plasmid

   #201102

   Purpose

   expression of human EGFR receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human EGFR receptor tyrosine kinase, full length, wildtype (EGFR Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid

    #50461

    Purpose

    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-mCherry (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    EF1a

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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