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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3,922 results for: pCMV mammalian

Showing: 3521 - 3540 of 3922 results

  1. NODAL-MYC-DYK-N72A-N199A

    Plasmid
    #115252
    Purpose
    For mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    N72A, N199A
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115252-plasmid-map-sequence-id-222234

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  2. NODALvar-MYC-DYK-C302S

    Plasmid
    #115254
    Purpose
    For mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    C302S
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115254-plasmid-map-sequence-id-222242

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  3. NODALvar-MYC-DYK-C302A

    Plasmid
    #115255
    Purpose
    For mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    C302A
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115255-plasmid-map-sequence-id-222245

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  4. NODALvar-MYC-DYK-N328A

    Plasmid
    #115246
    Purpose
    For mammalian expression of a human NODAL splice variant open reading frame (with a mutated N-glycosylation site) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL splice variant (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    N328A
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115246-plasmid-map-sequence-id-222166

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  5. CMV-hNET

    Plasmid
    #32811
    Depositor
    Susan Amara
    Insert
    NET (SLC6A2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only

  6. CMV-hEAAT4

    Plasmid
    #32816
    Depositor
    Susan Amara
    Insert
    hEAAT4 (SLC1A6 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)

    Plasmid
    #223106
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223106-plasmid-map-sequence-id-447626

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  12. pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)

    Plasmid
    #223107
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223107-plasmid-map-sequence-id-447623

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  13. pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)

    Plasmid
    #223108
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223108-plasmid-map-sequence-id-447631

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  14. pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)

    Plasmid
    #223109
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223109-plasmid-map-sequence-id-447628

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  16. CMV-hEAAT3

    Plasmid
    #32815
    Depositor
    Susan Amara
    Insert
    hEAAT3 (SLC1A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only
    32815-plasmid-map-sequence-id-456466

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Showing: 3521 - 3540 of 3922 results