We narrowed to 6,294 results for: nls

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iF51 PB-Zim3-dCas9-mScarlet-Hygro-Neo-SnapTag

Plasmid

#204725

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Tad-CBE2.4

Plasmid

#235055

Purpose

Expresses Tad-CBE2.4 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE2.4-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.6

Plasmid

#235058

Purpose

Expresses Tad-CBE3.6 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.6-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tol2_8xCTS-ECFP

Plasmid

#200249

Purpose

Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

8xCTS-ECFP

Use

Zebrafish expression

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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nCas9-NG_P2A-mCherry

Plasmid

#219934

Purpose

Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Sp-nCas9(D10A)-NG_P2A-mCherry

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

nCas9(D10A)

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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JAB2497

Plasmid

#190378

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::QacR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::QacR-NLS_19St

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGC247

Plasmid

#19634

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Tags

4xNLS

Expression

Worm

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pGC245

Plasmid

#19638

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R4-R3)

Use

Flp/frt, destination vector

Tags

4xNLS

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pME463

Plasmid

#238103

Purpose

Expressed ME-ABE ABE7.10-HK with SpRYCas9

Depositor

Alexis Komor

Insert

TadA*7.10(Y123H/N157K)-m_SpRYnCas9(D10A)

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

TadA modifications include Y123H, N157K; SpRY-nCa…

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pME234

Plasmid

#238104

Purpose

Expresses ME-ABE ABE7.10-HK with SpCas9 (NG

Depositor

Alexis Komor

Insert

TadA*7.10(Y123H/N157K)-m_SpnCas9(D10A)

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

TadA modifications include Y123H, N157K; NG-nCas9…

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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Tad-CBE2.1

Plasmid

#235053

Purpose

Expresses Tad-CBE2.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE2.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE2.5

Plasmid

#235054

Purpose

Expresses Tad-CABE2.5 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE2.5-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.1

Plasmid

#235056

Purpose

Expresses Tad-CBE3.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.2

Plasmid

#235057

Purpose

Expresses Tad-CBE3.2 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.2-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Tad-CABE1.1

Plasmid

#235051

Purpose

Expresses Tad-CABE1.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE1.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE1.2

Plasmid

#235052

Purpose

Expresses Tad-CABE1.2 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE1.2-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMO819

Plasmid

#232072

Purpose

Expresses Cytosine Base Editor for Mammalian Cells

Depositor

Franz Meitinger

Article

Ng et al Methods Mol Biol. 2025;2872:167-187. doi

Insert

Cytosine Base Editor

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1-alpha core promoter

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMO1170

Plasmid

#232073

Purpose

Expresses Adenine Base Editor for Mammalian Cells

Depositor

Franz Meitinger

Article

Ng et al Methods Mol Biol. 2025;2872:167-187. doi

Insert

Adenine Base Editor

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1-alpha core promoter

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2488

Plasmid

#190373

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::IcaR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::IcaR-NLS_19St

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2487

Plasmid

#190372

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-NLS_19St

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2514

Plasmid

#190382

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::TetR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::TetR-NLS_19St

Expression

Plant

Available Since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2519

Plasmid

#190385

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::BetI-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::BetI-NLS_19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2518

Plasmid

#190384

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::RpaR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::RpaR-NLS_19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB465

Plasmid

#185098

Purpose

NMA111-GFP with NLS1 deleted under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111DNLS1-GFP

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB467

Plasmid

#185100

Purpose

NMA111-GFP with NLS2 deleted under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111DNLS2-GFP

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC183

Plasmid

#19630

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
PE-P3-maize

Plasmid

#185611

Purpose

For plant prime editing in maize plants

Depositor

Jinxiao Yang

Article

Xu et al Nat Plants. 2022 Jan;8(1):45-52. doi: 10

Insert

3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS

Use

CRISPR

Promoter

ZmUbi

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TO-hNGN2-BSD-mApple

Plasmid

#182308

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

hNGN2 (NEUROG2 Human)
bsd-T2A-mycNLS-mApple

Tags

T2A-mycNLS-mApple

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

Plasmid

#128424

Purpose

Human Codon Optimized dCas9-DNMT3A3L

Depositor

Bradley Bernstein

Article

Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

Insert

dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

T7 Promoter

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Tad-CBE(TCA3)

Plasmid

#235064

Purpose

Expresses Tad-CBE(TCA3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCA3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge