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plIVMD118

Plasmid

#160938

Purpose

Excess Capsid Protein in vivo mRNA display

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mCherry-FLAG tag

Tags

MYC tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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plIVMD115

Plasmid

#160937

Purpose

Excess Capsid Protein for in vivo mRNA display

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mCherry-MYC tag

Tags

MYC tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAM1160c2

Plasmid

#159724

Purpose

Express MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Tags

Gal4DBD and MPGIL-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAM1674

Plasmid

#159726

Purpose

Express MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Tags

Gal4DBD and MPTLV-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAM1479a3

Plasmid

#159725

Purpose

Express MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Tags

Gal4DBD and MYITV-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCSJ392

Plasmid

#159722

Purpose

Express Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

GID10 (GID10 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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GtCCR1_EYFP_pcDNA3.1

Plasmid

#159120

Purpose

This plasmid encodes GtCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

Insert

GtCCR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGAD-GH-Mefv iso1

Plasmid

#92107

Purpose

Yeast two hybrid

Depositor

Feng Shao

Article

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

Mefv isoform1 (Mefv Mouse)

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-Metavinculin tail

Plasmid

#107152

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Proc Natl Acad Sci U S A. 2016 Aug 23;11

Insert

metavinculin (VCL Human)

Tags

(His)8

Expression

Bacterial

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pETconNK-LGK

Plasmid

#81165

Purpose

pETconNK with levoglucosan kinase

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

Levoglucosan kinase wild-type

Tags

c-myc

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJexpress414_OmpA171_R4cons_Y129H

Plasmid

#64302

Purpose

Conservative version of OmpA Redesign 4 with Y129H mutation

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons_Y129H

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

25 mutations to lipid-facing surface

Promoter

T7

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H3478

Plasmid

#64292

Purpose

OmpA redesign hybrid with wild-type strands 3478

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H3478

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H346

Plasmid

#64312

Purpose

OmpA redesign hybrid with wild-type strands 346

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H346

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pCVD453

Plasmid

#66154

Purpose

cloning of E. coli E2348/69 left end of the LEE region, contains rorf1 and espG.

Depositor

James Kaper

Article

McDaniel et al Proc Natl Acad Sci U S A. 1995 Feb 28;92

Insert

LEE probe A rorf1, espG

Use

Cloning vector

Promoter

none

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H-78

Plasmid

#64313

Purpose

OmpA redesign hybrid with wild-type strands 123456

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H-78

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H-46

Plasmid

#64311

Purpose

OmpA redesign hybrid with wild-type strands 123578

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H-46

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_R4cons_P121F

Plasmid

#64301

Purpose

Conservative version of OmpA Redesign 4 with P121F mutation

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons_P121F

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_R4cons_G99I

Plasmid

#64300

Purpose

Conservative version of OmpA Redesign 4 with G99I mutation

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons_G99I

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_R4cons_G125L

Plasmid

#64298

Purpose

Conservative version of OmpA Redesign 4 with G125L mutation

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons_G125L

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H1358

Plasmid

#64297

Purpose

OmpA redesign hybrid with wild-type strands 1358

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H1358

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only