-
Plasmid#128387PurposeExpresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruptionDepositorUseLentiviral, Luciferase, and Synthetic BiologyTagsCLuc394 and NLuc398ExpressionMammalianMutationPromoterEFS and IRESAvailable sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only
-
MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP
Plasmid#75122PurposeRetroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tagDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTags3x FLAG-tagExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a
Plasmid#177435PurposeTo express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.DepositorInsertPhosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-I-Sce1-eno2:Tau-IRES-egfp
Plasmid#112205PurposeUsed to make transgenic zebrafish expressing human 4R/0N Tau under the neuronal eno2 promoterDepositorInsertMAPT (MAPT Human)
UseZebrafish expressionTagseno2 exon 2 translational fusion, ires-egfp at c …ExpressionMutationPromotereno2 from zebrafishAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST Halo-flag-CAD
Plasmid#188117PurposeExpresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tag and Halo tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimi…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2
Plasmid#113889PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2DepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
mGluR6 (200bp crit)+SV40p-tdTomato-IRES-AP
Plasmid#18810DepositorInsertmGluR6 (200bp crit)+SV40p-tdTomato-IRES-AP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-CD4-TLR4 cyto
Plasmid#133064PurposeRetroviral expression of mouse CD4 extracellular domain fused with the cytosolic tail of TLR4DepositorInsertCD4-TLR4cyto (Tlr4 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-NGN2-IRES-GFP-T2A-Sox11
Plasmid#90213PurposeTo convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.DepositorUseRetroviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-TLR4 delta tail
Plasmid#133063PurposeRetroviral expression of mouse TLR4 with out its cytosolic tailDepositorInsertTLR4 delta tail (Tlr4 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only