We narrowed to 9,187 results for: Pol;

1. pY71-tet.I.-1

   Plasmid

   #133539

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pY71-tet.I.25

   Plasmid

   #133538

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pY71-tet.I.20

   Plasmid

   #133537

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pY71-tet.I.14

   Plasmid

   #133535

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 17 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pY71-tet.I.13

   Plasmid

   #133534

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 16 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pY71-tet.I.12

   Plasmid

   #133533

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 15 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pY71-tet.I.11

   Plasmid

   #133532

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 14 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pY71-tet.I.10

   Plasmid

   #133531

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 13 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pY71-tet.I.9

   Plasmid

   #133530

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 12 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pY71-tet.I.7

    Plasmid

    #133529

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 10 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pY71-tet.I.6

    Plasmid

    #133528

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 9 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pY71-tet.I.5

    Plasmid

    #133527

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 8 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pY71-tet.I.3

    Plasmid

    #133525

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 6 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pY71-tet.I.2

    Plasmid

    #133524

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 5 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pY71-tet.I.4

    Plasmid

    #133526

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 7 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pY71-tet.I.1

    Plasmid

    #133523

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFE-dabAB2 C539A

    Plasmid

    #133008

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene  (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Mutation

    C539A

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFE-dabAB2 H524A

    Plasmid

    #133007

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a H524A mutation and a dabB2 gene (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Mutation

    H524A

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFE-dabAB2 D353A

    Plasmid

    #133006

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene  (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Mutation

    D353A

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15b-GBdm

    Plasmid

    #129690

    Purpose

    express E. coli GreB E82C/C68S

    Depositor

    Jeff Gelles

    Article

    Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GreB E82C C68S (greB E. coli)
    

    Tags

    his6

    Expression

    Bacterial

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMH-HT_SsCHAD-H29A-R32A-R36A

    Plasmid

    #127786

    Purpose

    Expresses a SsCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    mutations in His29, Arg32 and Arg36 to Ala

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMH-HT_CtCHAD-R438A-K441A

    Plasmid

    #127791

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in Arg4…

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMH-HT_ygiF-TTM

    Plasmid

    #127794

    Purpose

    Expresses the TTM/CYTH domain of ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF (ygiF Escherichia coli)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    N-terminal TTM domain of ygiF (residues 1-200)

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEM-myr-Citrine-SV40pA-FKF

    Plasmid

    #119865

    Purpose

    Donor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    myr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    2x myristoylation

    Expression

    Bacterial

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRPB1–4stop

    Plasmid

    #112824

    Purpose

    pSMF2 with repeats 8–10 interrupted with 4 repeats containing stop codons

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    pSMF2 with repeats 8–10 interrupted with 4 repeat…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p5A1-T

    Plasmid

    #92017

    Purpose

    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with 3 TEV cleavage sites
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Available Since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p5C3-T

    Plasmid

    #92022

    Purpose

    Expresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting sucA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FLAG-mPar6a K19A

    Plasmid

    #86149

    Purpose

    FLAG-mPar6a K19A - Does not bind to aPKC

    Depositor

    Senthil Muthuswamy

    Article

    Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

    Insert

    Par6 (Pard6a Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lysine to Alanine mutation K19A

    Promoter

    LTR

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

29. pCellFree_G03 STAT1

    Plasmid

    #67116

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pCellFree_G03 GTF2A1L

    Plasmid

    #67096

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2A1L (GTF2A1L Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only