We narrowed to 9,959 results for: Met
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Plasmid#65044PurposeExpresses Catalytically inactive GFP-PRMT7 LDIG(69-72)AAAA mutantDepositorInsertPRMT7 (Prmt7 Mouse)
UseBacterial propagationTagsGFPExpressionMammalianMutationL69A D70A I71A G72APromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRE3GV-SpdCas9-Dnmt3A/3L-WT
Plasmid#222502PurposeExpresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherryDepositorInsertsS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
mCherry
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterTRE3GV and hPGKAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX307/PTPN11 T73I
Plasmid#140939Purposeexpresses PTPN11 T73IDepositorInsertPTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
TagsV5ExpressionMammalianMutationT73IPromoterEF1aAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNUTN6His Y426F/Y517F hTFNG
Plasmid#70084PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobeDepositorInsertmutated human serum transferrin (TF Human)
TagsHexa His tag and N-terminal signal peptide, 4 aa …ExpressionMammalianMutationAsn413 Asp, Asn611Asp, Ty426Phe, Tyr517PhePromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His Y95F/Y188F hTFNG
Plasmid#69601PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobeDepositorInsertmutated human serum transferrin (TF Human)
TagsHexa His tag and N-terminal signal peptide, 4 aa …ExpressionMammalianMutationAsn413 Asp, Asn611Asp, Tyr95Phe, Tyr188PhePromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-REX-GECO0.9
Plasmid#61249PurposeExpresses REX-GECO0.9 in neuronsDepositorInsertREX-GECO0.9
UseAAVExpressionMammalianMutationSubstitutions relative to R-GECO1: P60R, V61W, R6…PromoterhSynAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ppst93-MCRmar-NG
Plasmid#192766PurposeExpresses NG-tagged MCRmar in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available sinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM6_Ptrc_BsMdh_BmHps_BmPhi
Plasmid#129104PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicusDepositorInsertBsMDH BmHPS BmPHI
ExpressionBacterialAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoBRET PD-1/SHP2 Bidirectional Vector
Plasmid#237016PurposeExpress NanoLuc(R)-PD1/SHP2-HaloTag Fusion Proteins in Mammalian Cells under a CMV promoterDepositorHas serviceDNATagsHaloTag (R) and NanoLuc (R)ExpressionMammalianMutationNonePromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
p1.2-GS-eGFP
Plasmid#162771PurposeFluorescent reporter for CHO expression studiesDepositorInsertenhanced green fluorescent protein
ExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His K206E hTFNG
Plasmid#70130PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobeDepositorInsertmutated human serum transferrin (TF Human)
TagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationchanged Lys206 to a glutamic acid, Asn413Asp, Asn…PromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D
Plasmid#159541PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G12D (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12DAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3-YFP
Plasmid#50484Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
TagsYFPExpressionMammalianMutationYFP fusion in M3-M4 loop (after residue P379). O…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc
Plasmid#129105PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone productionDepositorInsertBsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
ExpressionBacterialAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC_h7SK Mma PylT (CUA)
Plasmid#154782Purposeplasmid with single copy of Mma PylT (CUA anticodon)DepositorInsertMma PylT
ExpressionMammalianMutationU25CPromoterh7SKAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM066
Plasmid#225524PurposeEncodes Wnt-responsive destablized iRFPDepositorInsertiRFP
ExpressionMammalianAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXP120
Plasmid#26826DepositorTypeEmpty backboneExpressionYeastAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pALS3-Ma-SUMO-sfGFP-WT
Plasmid#212122PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)
TagsHis6 and SUMOExpressionBacterialPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHM092
Plasmid#225526PurposeEncodes rtTA under AR8 promoter, and constitutive BFPDepositorInsertsrtTA
BFP
ExpressionMammalianAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only