We narrowed to 18,451 results for: Met

1. SNF-5 pXOOM

   Plasmid

   #48697

   Purpose

   Expressed SNF-5 in Xenopus oocytes or mammalian cells

   Depositor

   Dmitri Boudko

   Article

   Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

   Insert

   snf-5 (snf-5 Nematode)
   

   Use

   Xenopus oocyte expression

   Expression

   Mammalian

   Mutation

   K42E (when compared to NP_496735.1)

   Promoter

   T7

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. POT2-RFP

   Plasmid

   #65318

   Purpose

   receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   RFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Clover-PDHA1-N-10

   Plasmid

   #56304

   Purpose

   Localization: Mitochondria, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   PDHA1 (PDHA1 Human)
   

   Tags

   Clover

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pMSP1D1G1

   Plasmid

   #97325

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Article

   Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

   Insert

   MSP1D1G1
   

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of G residue after T25

   Promoter

   T7

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro

   Plasmid

   #31480

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (ani target)
   

   Use

   Lentiviral

   Mutation

   mCherry has M9S, M16L to reduce background mCherr…

   Available Since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFAB815

   Plasmid

   #47854

   Purpose

   BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   rnpB T1 terminator
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLTetO9

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFU-MVIIA-PE

   Plasmid

   #24144

   Depositor

   Ines Ibanez-Tallon

   Article

   Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

   Insert

   omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMV-HIS3

   Plasmid

   #65333

   Purpose

   standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUs

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   HIS3
   

   Use

   Synthetic Biology

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-ybbR-HIS-Xyl-DocI

   Plasmid

   #58711

   Purpose

   Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   Xyl-DocI
   

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   changed Threonin 120 to Cysteine in Xylanase Doma…

   Promoter

   T7

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. peCALWY-2

    Plasmid

    #22234

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-2 Zn(II) sensor (ATOX1 Human, GFP's)
    

    Expression

    Mammalian

    Mutation

    sequence identical to eCALWY-1, but linkerlength …

    Available Since

    Nov. 20, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pEN-X-hPGK-PuroR-Z

    Plasmid

    #218904

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    hPGK-PuroR
    

    Expression

    Mammalian

    Mutation

    246T>A and 398T>C

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDuet-1_VNp15-DARPinOFF7

    Plasmid

    #182399

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-DARPinOFF7
    

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ1208

    Plasmid

    #44490

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]
    

    Use

    Gateway expression clone

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-pelB-LaG-17

    Plasmid

    #172747

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-17
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMT-Map7-1333-end

    Plasmid

    #46070

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-1333-end (Map7 Mouse)
    

    Expression

    Bacterial

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. mIFP-Lysosomes-20

    Plasmid

    #56228

    Purpose

    Localization: Lysosome Membrane, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    LAMP1
    

    Tags

    mIFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. piGECI-NES-N1

    Plasmid

    #160422

    Purpose

    Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI-NES
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. mRuby2-Nup50-N-10

    Plasmid

    #55908

    Purpose

    Localization: Nuclear Pore, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Nup50 (NUP50 Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. eGFP-RAPH1-F1

    Plasmid

    #194860

    Purpose

    Express the RAPH1 fragment (1-535) tagged to eGFP in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acids 1-535

    Promoter

    CMV

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXS-IRES-Blast SHMT1

    Plasmid

    #106304

    Purpose

    Express SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 1 (SHMT1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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