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pET28-GG-PUF

Plasmid

#52137

Purpose

Receiving vector for PUM-HD assembly and expression in E.coli

Depositor

Huimin Zhao

Publication

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Type

Empty backbone

Tags

PUM-HD flanking region 1' and PUM-HD flankin…

Expression

Bacterial

Promoter

T7

Available since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCITETtTERT

Plasmid

#27660

Depositor

Kathleen Collins

Publication

Prathapam et al Nat Struct Mol Biol. 2005 Mar . 12(3):25

Insert

Tetrahymena TERT

Use

Reticulocyte lysate

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
C125-E01: stuffer

Plasmid

#162900

Purpose

Standard Gateway entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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lvl0 UBQ10p

Plasmid

#153363

Purpose

Plant promoter

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

UBQ10 promoter

Expression

Plant

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 UBQ11p

Plasmid

#153365

Purpose

Plant promoter

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

UBQ11 promoter

Expression

Plant

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Muc2D1D2D3

Plasmid

#155215

Purpose

Expresses human MUC2 residues 21-1259

Depositor

Deborah Fass

Publication

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D1D2D3 (MUC2 Human)

Tags

6xHistadine

Expression

Mammalian

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK107

Plasmid

#110575

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP25 Promoter with RBS

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK121

Plasmid

#110581

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

PA3 Promoter with RBS

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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LI C-D 1-10GFP

Plasmid

#54461

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

superfolder GFP 1-10 domain

Use

Cloning vector

Available since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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LI E-F nos-T c2

Plasmid

#54407

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Nopaline synthase terminator

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pLPO1

Plasmid

#209975

Purpose

Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (256_rep)

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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TRE-mClover3

Plasmid

#214922

Purpose

expression vector control - contitutive expression of mClover3 alone

Depositor

Ophir Shalem

Publication

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

mClover3

Use

Lentiviral

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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Skp

Plasmid

#213139

Purpose

Expresses chaperone protein SKP

Depositor

Sebastian Hiller

Publication

Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

Insert

SKP (21-161) (skp E.coli)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP-DQNAT

Plasmid

#198210

Purpose

pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Publication

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

sfGFP_DQNAT

Tags

6x His Tag and DQNAT glycosylation tag

Expression

Bacterial

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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MBP-RGG-SmBit-RGG

Plasmid

#176644

Purpose

SmBit fused to LAF-1 RGG domain with N-terminal MBP tag

Depositor

Daniel Hammer

Publication

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

MBP-RGG-SmBit-RGG

Expression

Bacterial

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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C121-E02: ffLuc2

Plasmid

#162889

Purpose

Standard Gateway entry clone encoding firefly luciferase

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Firefly luciferase2

Use

Synthetic Biology

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDest-676 (attR4-attR3)

Plasmid

#161888

Purpose

Multisite Gateway (attR4-attR3) vector for lentivirus production

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK403

Plasmid

#110599

Purpose

BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

mRFP dropout (RSF1010 origin)

Use

Synthetic Biology

Available since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pNS1

Plasmid

#131769

Purpose

PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Publication

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

FUSN_Fusion Red_Cry2WT

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTK750

Plasmid

#114718

Purpose

Express mCherry-NuMA(5A-3) from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NuMA 5A-3

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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