We narrowed to 31,032 results for: uros

1. 1053 pBabe puroL Akt K179M T308A S473A

   Plasmid

   #9013

   Depositor

   William Sellers

   Article

   DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

   Insert

   Akt1 K179M T308A S473A (AKT1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K179M T308A S473A

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

2. pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro

   Plasmid

   #210605

   Purpose

   heat shock promoter - activated hyperdCas12a-based transcriptional base editor

   Depositor

   Stanley Qi

   Article

   Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

   Insert

   ABE8e-hyperdCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   truncated hsp70

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

   Plasmid

   #213118

   Purpose

   Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   PKAsub-mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. XLone-Puro CCND2-T2A-luciferase-P2A-eGFP

   Plasmid

   #179843

   Purpose

   Tunable and temporal expression control of CCND2, luciferase and nuclear eGFP

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Luciferase and Synthetic Biology

   Tags

   eGFP and luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro

   Plasmid

   #170467

   Purpose

   The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Bakhache et al bioRxiv 2021

   Insert

   Nucleocapsid
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. RRL.sin.cPPT.SFFV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG536)

   Plasmid

   #139443

   Purpose

   Lentiviral vector to ectopically express human NCOA7 isoform 4 from SFFV promoter

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

   Plasmid

   #154399

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP

   Depositor

   Xiaoping Bao

   Insert

   SARS-CoV2 N Protein (N SARS-CoV2)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR

   Plasmid

   #171963

   Purpose

   Donor plasmid for CLTX T-CAR expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

   Insert

   Synthetic CLTX CD32a-tm CD3z CAR
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro

   Plasmid

   #120560

   Purpose

   Expresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   HRAS (HRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   CMV

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)

    Plasmid

    #214877

    Purpose

    Lentiviral vector encoding RfxCas13d targeting LPT guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crLPT-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAVS1-Puro CAG-CLTX CD32a ITAM CAR

    Plasmid

    #171964

    Purpose

    Donor plasmid for CLTX CD32a-ITAM CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

    Insert

    Synthetic CLTX CD32a ITAM CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE N-mRuby3 (from SARS-CoV-2) IRES puro

    Plasmid

    #170466

    Purpose

    The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mRuby3 fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Bakhache et al bioRxiv 2021

    Insert

    Nucleocapsid
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101101

    Purpose

    Encodes dCas9(N) fused to transmembrane tether

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    TMt-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3-MTS-CCDB-N136-RsDddA-A-UGI-PURO

    Plasmid

    #205463

    Purpose

    A backbone containing a RsDddA fragment A for constructing mitochondria-targeted RsDdCBE expression plasmid

    Depositor

    Bin Shen

    Article

    Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

    Insert

    MTS; N136; ccdb; RsDddA-A; UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-MTS-CCDB-N136-RsDddA-C-UGI-PURO

    Plasmid

    #205465

    Purpose

    A backbone containing a RsDddA fragment C for constructing mitochondria-targeted RsDdCBE expression plasmid

    Depositor

    Bin Shen

    Article

    Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

    Insert

    MTS; N136; ccdb; RsDddA-C; UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

    Plasmid

    #157985

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-CMV-Flag-PolB(K244A/T304I)-Puro

    Plasmid

    #177144

    Purpose

    Lentiviral vector expressing Flag-PolB(K244A/T304I) and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

    Plasmid

    #120564

    Purpose

    Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-IRES-puro-NLS-dHgm4Cas13b-EGFP-NLS

    Plasmid

    #191360

    Purpose

    Overexpression of NLS-dHgm4Cas13b-EGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dHgm4Cas13b-EGFP-NLS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101108

    Purpose

    Encodes dCas9(N) fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge