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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,415 results for: Coli

Showing: 8901 - 8950 of 9415 results

  2. ARK1a

    Plasmid
    #81016
    Purpose
    E.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)
    Depositor
    Taek Soon Lee
    Article
    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10
    Inserts
    MevTo-BBa1002-pTrc-Mkco-PMKco
    PMD-NudB
    Use
    Tags
    Expression
    Mutation
    Promoter
    LacUV5 and Trc promoter
    Available Since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81016-plasmid-map-sequence-id-153403

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  13. pDXinit

    Plasmid
    #110101
    Purpose
    E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages
    Depositor
    Markus Seeger
    Article
    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110101-plasmid-map-sequence-id-211288

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  18. pT22-GluCNZ-6His

    Plasmid
    #213504
    Purpose
    Designed for cytoplasmic E.coli overexpression of glucanase (Ī²-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.
    Depositor
    John Denu
    Article
    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12
    Insert
    Glucanase (glucan endo-1,3-beta-glucosidase)
    Use
    Tags
    6His tag
    Expression
    Bacterial
    Mutation
    1-36 Signal Sequence Deletion
    Promoter
    T7
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213504-plasmid-map-sequence-id-424932

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  26. pNICKclos1.0

    Plasmid
    #73639
    Purpose
    Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824
    Depositor
    Sheng Yang
    Article
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    Cas9 nickase
    sGRNA to pyrE
    Use
    E.coli-clostridium shuttle vector
    Tags
    Expression
    Mutation
    D10A
    Promoter
    j23119 and ptb
    Available Since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73639-plasmid-map-sequence-id-148603

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  29. pBXNPHM3

    Plasmid
    #110099
    Purpose
    E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage site
    Depositor
    Markus Seeger
    Article
    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1
    Type
    Empty backbone
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110099-plasmid-map-sequence-id-258124

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  32. pSUB11

    Plasmid
    #169699
    Purpose
    Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
    Use
    Tags
    3xFLAG tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169699-plasmid-map-sequence-id-336760

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  36. pBSV2G_P0826-BB0323-mCherryBb

    Plasmid
    #118244
    Purpose
    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter
    Depositor
    Christine Jacobs-Wagner
    Article
    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii
    Insert
    BB0323-mCherry translational fusion under the control of the bb0826 promoter
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118244-plasmid-map-sequence-id-231804

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  37. pSUB7

    Plasmid
    #169698
    Purpose
    Template plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169698-plasmid-map-sequence-id-334547

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  38. pSU315

    Plasmid
    #169697
    Purpose
    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
    Use
    Tags
    1xHA tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169697-plasmid-map-sequence-id-334518

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  41. pdCASclos

    Plasmid
    #73640
    Purpose
    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824
    Depositor
    Sheng Yang
    Article
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    dCas9
    sgRNA to spo0A
    Use
    E.coli-clostridium shuttle vector
    Tags
    Expression
    Mutation
    D10A, H840A
    Promoter
    Pj23119 and ptb
    Available Since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73640-plasmid-map-sequence-id-148604

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  44. pAD-SPS6-Pman

    Plasmid
    #133906
    Purpose
    Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.
    Depositor
    Oscar Kuipers
    Article
    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57
    Insert
    sfGFP(SPS6)
    Use
    E.coli/bacillus spp. shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pman
    Available Since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    133906-plasmid-map-sequence-id-261727

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Showing: 8901 - 8950 of 9415 results