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pGEX-GP-2-MPHOSPH8 Chromo

Plasmid

#59694

Purpose

Contains histone modification interacting domain (MPHOSPH8 Chromo)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

MPHOSPH8 Chromo (MPHOSPH8 Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5L18-CtermQ7

Plasmid

#51442

Purpose

Expresses TALEN targeting left CCR5A site (L18) with Q7 C-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Left (L18) Q7 C-term

Use

TALEN

Tags

3xFLAG and Fok1 EL variant

Expression

Mammalian

Mutation

K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pMIY-mETV3-Flag

Plasmid

#34579

Depositor

Peter Murray

Article

El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

Insert

Etv3 (Etv3 Mouse)

Use

Retroviral

Tags

FLAG and IRES-YFP

Expression

Mammalian

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only
MTK0_031

Plasmid

#123970

Purpose

Encodes the BxBI-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BxBI-hygro-mRuby2PEST landing

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_033

Plasmid

#123996

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA2 -hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_034

Plasmid

#123997

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA3 -hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad1 (4SP/AP)

Plasmid

#14043

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

Insert

Smad1 (4SP/AP) (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Four Serine to Alanine mutations in the linker re…

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
MTK7_014

Plasmid

#123881

Purpose

Encodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

3' HIV LTR (3

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3b_031

Plasmid

#123809

Purpose

Encodes Kruppel Associated Box repression domain fused to methyl CpG binding protein 2 as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

KRAB:meCP2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPuroR-MCS-LexA-VP16-MiniWhite

Plasmid

#165898

Purpose

Puromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pB-Neo-rTOM20-GFP

Plasmid

#210331

Purpose

Overexpression of rTOM20

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

rTOM20 (Tomm20 Rat)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSIN-Ski-gRNA2

Plasmid

#180369

Purpose

targeting mouse Ski gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Ski targeting gRNA (Ski Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Skil-gRNA2

Plasmid

#180371

Purpose

targeting mouse Skil/SnoN gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Skil targeting gRNA (Skil Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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PDF1::mCitrine-MBD

Plasmid

#115222

Purpose

Microtubule visualization

Depositor

Jan Traas

Article

Armezzani et al Development. 2018 Jun 4;145(11). pii: de

Insert

Microtubule binding domain

Tags

mCitrine

Expression

Plant

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5R18-CtermQ7

Plasmid

#51443

Purpose

Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) Q7 C-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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ATF4 10: 5’ATF4.uORF1.GFP

Plasmid

#21857

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

upstream ORF1 (uORF) of ATF4 fused to EGFP

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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MTK5_012

Plasmid

#123856

Purpose

Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector RE'-A1 Insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS4

Plasmid

#218713

Purpose

Expresses N-terminally EGFP-tagged BBS4 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS4 (BBS4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

I354T (t1110c)

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 48-232 (#182)

Plasmid

#99920

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

N-terminal deletion; encodes AA 48-232

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Cx43-sfGFP

Plasmid

#70053

Purpose

Expresses rat Cx43 (Gja1 CDS) tagged with non-monomerized sfGFP on the C-terminus. CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx43 (Gja1 Rat)

Tags

non-monomerized superfolder GFP (V207)

Mutation

fused in frame to non-monomerized superfolder GFP

Promoter

CMV

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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