We narrowed to 18,889 results for: Tec

1. mApple-VASP-5

   Plasmid

   #54958

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   VASP
   

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   A209T in VASP

   Promoter

   CMV

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCellFree_G03 SREBF2

   Plasmid

   #67129

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   SREBF2 (SREBF2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pTMS2

   Plasmid

   #87800

   Purpose

   For constructing MBP-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB5935

   Plasmid

   #106128

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntA_1, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 YY1

   Plasmid

   #67082

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   YY1 (YY1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLKO.1P shMOCS3_2

   Plasmid

   #102974

   Purpose

   Suppression of MOCS3

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shMOCS3_2 (MOCS3 Human)
   

   Use

   Lentiviral and RNAi

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1P shMOCS3_1

   Plasmid

   #102973

   Purpose

   Suppression of MOCS3

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shMOCS3_1 (MOCS3 Human)
   

   Use

   Lentiviral and RNAi

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. YSBE 628 : pHYG_mCherry

   Plasmid

   #92092

   Purpose

   Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, HYG selection

   Depositor

   Yong-Sun Bahn

   Article

   So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

   Insert

   mCherry
   

   Use

   tagging proteins in cryptococcus neoforman

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB2192

   Plasmid

   #67539

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-8xHis

    Plasmid

    #101699

    Purpose

    8xHis in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    AATG-[8xHis]-AGCC
    

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. YSBE 558 : pNAT_4xFLAG

    Plasmid

    #92086

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, NAT selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    4xFlag
    

    Use

    tagging proteins in cryptococcus neoforman

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pML2003

    Plasmid

    #193255

    Purpose

    Propionate sensor expressing eYFP reporter

    Depositor

    Lauren Andrews

    Article

    Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

    Inserts

    prpR
    eYFP
    

    Use

    Synthetic Biology

    Promoter

    Pprp and Synthetic promoter P6

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA4/TO-2xStrep-BFLF1

    Plasmid

    #162626

    Purpose

    Expresses N-terminal 2x-Strep-tagged BFLF1 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    BFLF1
    

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCoofy47

    Plasmid

    #55188

    Purpose

    Mammalian expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    10x His-Stop-OneStrep-Stop-S-tag-Stop-CBP-Stop-HP…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. GGE209

    Plasmid

    #120793

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp-Mfe
    

    Use

    Cloning/assembly plasmid

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCellFree_G03 SP6

    Plasmid

    #67074

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    SP6 (SP6 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. MSGV Rh Acceptor PGK-NGFR

    Plasmid

    #64272

    Purpose

    Retroviral golden gate cloning vector for expression of functional Rhesus T-Cell Receptor genes with NGFR

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TAL3512

    Plasmid

    #47231

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-pcdh10a-left (pcdh10a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pBT100.136c

    Plasmid

    #122603

    Purpose

    clone FERNY phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    FERNY
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFLIP37

    Plasmid

    #65505

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Tags

    6His-Cit-attR1 and attR2-Cer-cMyc

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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