We narrowed to 4,274 results for: sars

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pcDNA5-FRT-TO-Nsp2-HF

Plasmid

#157684

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp2-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR

Plasmid

#204579

Purpose

lentivirus backbone cloning vector and template for barcoded non-neutralizable standard production

Depositor

Jesse Bloom

Publication

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

mCherry

Use

Lentiviral

Promoter

TRE3G

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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TMPRSS2 g3

Plasmid

#153017

Purpose

A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry

Depositor

Jason Sheltzer

Insert

TMPRSS2 gRNA

Use

CRISPR and Lentiviral

Tags

mCherry

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMCSG53_Nsp1

Plasmid

#167256

Purpose

Bacterial expression of Nsp1 residues 13-127

Depositor

Alexei Savchenko

Publication

Semper et al iScience. 2021 Jan 22;24(1):101903. doi:

Insert

Nsp1 (residues 13-127) (ORF1ab SARS-CoV-2)

Tags

His6-TEV site

Expression

Bacterial

Mutation

none

Available since

April 27, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pMCSG53_N

Plasmid

#167255

Purpose

Bacterial expression of Nucleocapsid protein (residues 1-419)

Depositor

Alexei Savchenko

Insert

N protein (residues 1-419) (N SARS-CoV-2)

Tags

His6-TEV site

Expression

Bacterial

Mutation

none

Available since

April 27, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9

Plasmid

#231910

Purpose

For packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Publication

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc

Expression

Mammalian

Promoter

CMV

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-SC2Hel

Plasmid

#258844

Purpose

Expression of His-tagged SARS-CoV-2 Helicase nsp13 in E. coli

Depositor

Edward D'Antonio

Publication

et al BioChem 2024, 4, 115-125.

Insert

Helicase

Tags

KLAAALEHHHHHH and MGRGSHHHHHHGMA

Expression

Bacterial

Promoter

T7 lac

Available since

Aug. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

Plasmid

#176059

Purpose

Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

Depositor

Britt Glaunsinger

Publication

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)

Tags

3xFLAG

Expression

Mammalian

Mutation

R124A and K125A

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v2.4-IgG1

Plasmid

#149668

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.4

Depositor

Erik Procko

Publication

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-sACE2v2-sfGFP

Plasmid

#145173

Purpose

Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2

Depositor

Erik Procko

Publication

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Linker GSGGSGSGG and superfolder GFP

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p_L_3c_SG_RLuc

Plasmid

#208729

Purpose

For assessing leaky scanning rates in mammalian cells

Depositor

Andrew Firth

Insert

ORF3c (ORF3a )

Tags

Renilla luciferase

Expression

Mammalian

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+) NRP1 b1b2 (273-586)

Plasmid

#162733

Purpose

Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

Depositor

Brett Collins

Publication

Daly et al Science. 2020 Oct 20. pii: science.abd30

Insert

Human NRP1 b1b2 domains (residues 273-586)

Tags

6His

Expression

Bacterial

Mutation

Codon optimised

Available since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET303_sumocodo_GFP_codoopti

Plasmid

#210393

Purpose

Express SUMO-GFP in E. coli

Depositor

Magnus Rueping

Publication

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

GFP

Tags

SUMO-tag

Expression

Bacterial

Available since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-S-501V2-FLAG

Plasmid

#177096

Purpose

501V2 variant S-protein with c-terminal FLAG tag

Depositor

Daniel Conway

Insert

S-protein 501V2 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Available since

Nov. 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-S-P1-FLAG

Plasmid

#177095

Purpose

P1 variant S-protein with c-terminal FLAG tag

Depositor

Daniel Conway

Insert

S-protein P1 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Available since

Oct. 18, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBx1-VHH template1-3XMyc-Spacer

Plasmid

#176208

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Publication

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template1-3XMyc-Spacer

Tags

Myc tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCW-tTRE-scFv-SunTag

Plasmid

#193138

Purpose

Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

Depositor

Brad Rosenberg

Publication

Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

Insert

scFv-SunTag

Use

Lentiviral

Expression

Mammalian

Promoter

tight TRE promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-S-B.1.1.7-FLAG

Plasmid

#177093

Purpose

B.1.1.7 variant S-protein with c-terminal FLAG tag

Depositor

Daniel Conway

Insert

S-protein B.1.1.7 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Available since

Nov. 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-S-B.1.617.1-FLAG

Plasmid

#177098

Purpose

B.1.617.1 variant S-protein with c-terminal FLAG tag

Depositor

Daniel Conway

Insert

S-protein B.1.617.1 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Available since

Nov. 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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LgBiT-hACE2

Plasmid

#173431

Purpose

protein expression plasmid of LgBiT-hACE2-IgG1 Fc

Depositor

David Nemazee

Publication

Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

Insert

LgBiT-hACE2-IgG1 Fc (ACE2 Human)

Use

Luciferase

Tags

IgG1

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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