We narrowed to 9,424 results for: try

1. FUG-T2A-Pten

   Plasmid

   #140738

   Purpose

   Lentiviral vector to overexpress human PTEN and GFP

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-PTA-PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   hUbiC

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ262-ABE8e

   Plasmid

   #161523

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   ABE8e-zSpCas9(D10A)
   

   Use

   CRISPR; Gateway compatible abe8e-zspcas9(d10a) en…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpyTag-RBD

   Plasmid

   #159999

   Purpose

   Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFR-ET1

   Plasmid

   #44185

   Depositor

   Timothy Springer

   Article

   Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

   Insert

   EGFR-ET1 (EGFR Human)
   

   Use

   Tags

   3C cleavge site, protein C peptide tag, his6, SBP…

   Expression

   Mammalian

   Mutation

   I104V and D254N relative to NP_005219.2

   Promoter

   CMV

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCK069 desmaMCS:minprom_mApple,exorh:EGFP

   Plasmid

   #195982

   Purpose

   Expression construct

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   desmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR221-FGFR4

   Plasmid

   #60531

   Purpose

   Entry vector for human FGFR4

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   FGFR4 (FGFR4 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   c2134a P712T

   Promoter

   NA

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCR8/GW_TOPO_Cdk12_Untagged

   Plasmid

   #127176

   Purpose

   Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Entry vector with transgene for gateway cloning

   Tags

   None

   Expression

   Mutation

   Promoter

   None

   Available since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC-BSG

   Plasmid

   #158383

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   BSG (BSG Human)
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDONR223-TAF5-FL

   Plasmid

   #209047

   Purpose

   Gateway-compatible entry vector, with insert of the full length TAF5 gene

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 full length
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. scFv-GCN4-DNMT3a-DNMT3l

    Plasmid

    #154140

    Purpose

    Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Use

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCK033 p5E desma MCS

    Plasmid

    #195948

    Purpose

    Gateway p5E vector

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements (desma Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEN_TT 3xFLAG-KPNB1

    Plasmid

    #192301

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KPNB1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNB1 (KPNB1 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ266E

    Plasmid

    #161521

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    zSpRYCas9(D10A)-PmCDA1-UGI
    

    Use

    CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ265E6

    Plasmid

    #213469

    Purpose

    Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

    Depositor

    Yiping Qi

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Insert

    TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
    

    Use

    CRISPR; Gateway compatible tada-cda-32aa linker-z…

    Tags

    No

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. FAK2_HUMAN_D0

    Plasmid

    #79747

    Purpose

    This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    FAK2 (PTK2B Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pYPQ262B-ABE8e

    Plasmid

    #161524

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    TadA8e-zSpRYCas9(D10A)
    

    Use

    CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB-hCMV1-hKLF2-IV

    Plasmid

    #60433

    Purpose

    tet-inducible human KLF4

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Insert

    KLF2 (KLF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA

    Plasmid

    #104097

    Purpose

    Expresses TREAT-PK co-localization control in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xMS2 stem loops in 3'UTR and 24xPP7 stem l…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDule2-3-nitroTyrosine (5B)

    Plasmid

    #85499

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21b-Caspase-3(D3A: D175A D28A D9A)

    Plasmid

    #90090

    Purpose

    Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

    Depositor

    Clay Clark

    Article

    Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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