We narrowed to 9,512 results for: try

1. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

   Plasmid

   #154139

   Purpose

   Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
   

   Tags

   3x FLAG and tagBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. NowGFP /pQE-30

   Plasmid

   #74749

   Purpose

   NowGFP gene for bacterial expression

   Depositor

   Konstantin Lukyanov

   Article

   Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

   Insert

   NowGFP
   

   Tags

   HisTag (with a mutation but still working)

   Expression

   Bacterial

   Mutation

   WasCFP with substitutions E6K, L42M, T43S, V68M, …

   Promoter

   T5

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. mTq2-Calmodulin

   Plasmid

   #198200

   Purpose

   FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   Calmodulin 2 (Calm2 Rat)
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIVT-R2Tg-RTmut

   Plasmid

   #238106

   Purpose

   For IVT of RT-mutant R2Tg editor, codon optimized for mammalian expression.

   Depositor

   Feng Zhang

   Article

   Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

   Insert

   Reverse transcriptase mutant R2Tg, mammalian codon optimized
   

   Use

   For ivt

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUG-Pten

   Plasmid

   #140732

   Purpose

   Lentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion protein

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-Pten (PTEN Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   hUbiC

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR1a-tagRFP-KDEL

   Plasmid

   #114177

   Purpose

   Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

   Depositor

   Nicolas Demaurex

   Article

   Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

   Insert

   tagRFP-KDEL
   

   Use

   Gateway entry clone

   Tags

   KDEL retention signal and signal peptide

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBpaRS3tRNA

   Plasmid

   #197102

   Purpose

   To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

   Insert

   TyrRS variant, amber suppressor tRNA
   

   Expression

   Bacterial

   Mutation

   Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-GABARAPL2

   Plasmid

   #137761

   Purpose

   Expression of GABARAPL2

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   GABARAPL2 (GABARAPL2 Human)
   

   Tags

   HA and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. phage UbiC NLS HA stdPCP stdGFP

   Plasmid

   #104099

   Purpose

   Lentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   stdPCP-stdGFP
   

   Use

   Lentiviral

   Tags

   N-terminal NLS, Two synonymous copies of GFP (C-t…

   Expression

   Mammalian

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

    Plasmid

    #127354

    Purpose

    gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-GABARAPL1

    Plasmid

    #137759

    Purpose

    Expression of GABARAPL1

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    GABARAPL1 (GABARAPL1 Human)
    

    Tags

    HA and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. plentiCMV10x-sct-pp65-b2m-HLA-A0201

    Plasmid

    #196513

    Purpose

    Express single-chain pMHC with sequencing tag for 10x genomics

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    single chain of a pp65 peptide, b2m and HLA A0201
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Y84C, 10xgenomics compatible tag

    Promoter

    CMV

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. phage UbiC NLS HA stdMCP Halo

    Plasmid

    #104098

    Purpose

    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    stdMCP-Halo
    

    Use

    Lentiviral

    Tags

    C-terminal Halo-tag, N-terminal NLS, and Two syno…

    Expression

    Mammalian

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST47-CYFIP1.GFP

    Plasmid

    #109139

    Purpose

    GFP-tagged CYFIP1 expression construct

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDule-3-nitroTyrosine (5B)

    Plasmid

    #85498

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. VRK1_HUMAN_D0

    Plasmid

    #79684

    Purpose

    This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    VRK1 (VRK1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. HA-MAP1LC3A

    Plasmid

    #137756

    Purpose

    Expression of LC3A

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    MAP1LC3A (MAP1LC3A Human)
    

    Tags

    HA and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAcBac1-Pyl-TAG

    Plasmid

    #228784

    Purpose

    Pyl aaRS/tRNA system for mammalian TAG suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    PylRS
    PytR
    

    Use

    Baculoviral

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA-mSin1(1.1)-HA

    Plasmid

    #73388

    Purpose

    Expresses mSin-HA

    Depositor

    Jie Chen, Taekjip Ha

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    mSin1 transcript variant protein isoform 1 (MAPKAP1 Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCF160_VSV-G

    Plasmid

    #225963

    Purpose

    CMV-Intron-VSVG (env protein). Expresses VSV-G env for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-VSVG (env protein)
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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