We narrowed to 625 results for: PK1

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pLEX307_PDHA2S291D/S293D

Plasmid

#115195

Purpose

Lentiviral transduction and expression of PDHA2S291D/S293D into any mammalian cell

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S291D/S293D (PDHA2 Human)

Use

Lentiviral

Mutation

p.S291D/S293D

Promoter

EF1alpha

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-ERK2 GOF

Plasmid

#53174

Purpose

Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human, Mustard Weed)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R67S/D321N

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pLentiPGK Puro DEST JNKKTRClover

Plasmid

#59151

Purpose

Lentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp BFP-Erk D319N

Plasmid

#102350

Purpose

Lentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutation

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

Erk2 (Mapk1 Mouse)

Use

Lentiviral

Tags

BFP

Mutation

Aspartic acid 319 was mutated to asparagine

Promoter

SFFV

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cn-VA-NHEJ1-WT

Plasmid

#141339

Purpose

Lentiviral vector for expression of human NHEJ1-WT in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1 (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-HA-ERK2 K54R

Plasmid

#53170

Purpose

Expresses GST-HA tagged human ERK2 K54R from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human, Mustard Weed)

Tags

GST and HA

Expression

Bacterial

Mutation

K54R

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-ERK2 R67S

Plasmid

#53172

Purpose

Expresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human, Mustard Weed)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R67S

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-ERK2 D321N

Plasmid

#53173

Purpose

Expresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human, Mustard Weed)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

D321N

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag p38 alpha(agf)

Plasmid

#20787

Depositor

Roger Davis

Article

Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

Insert

pCMV5 Flag p38 alpha (agf) (Mapk14 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

replaced Thr180 and Tyr182 with Ala and Phe, resp…

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
MK11_HUMAN_D0

Plasmid

#79692

Purpose

This plasmid encodes the kinase domain of MK11. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK11 (MAPK11 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pLD-puro-Cn-VA-NHEJ1-S263E

Plasmid

#141340

Purpose

Lentiviral vector for expression of human NHEJ1-S263E in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1-S263E (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Mutation

S263E

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cn-VA-NHEJ1-S263A

Plasmid

#141341

Purpose

Lentiviral vector for expression of human NHEJ1-S263A in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1-S263A (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Mutation

S263A

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST p38KTRmCerulean3

Plasmid

#59155

Purpose

Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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MAPK8 gRNA (BRDN0001146407)

Plasmid

#78048

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK8 (MAPK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK8 gRNA (BRDN0001147976)

Plasmid

#78049

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK8 (MAPK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK8 gRNA (BRDN0001487083)

Plasmid

#78047

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK8 (MAPK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK8 gRNA (BRDN0001144736)

Plasmid

#78046

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK8 (MAPK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MK08_HUMAN_D0

Plasmid

#79695

Purpose

This plasmid encodes the kinase domain of MK08. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK08 (MAPK8 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pLD-puro-Cn-VA-NHEJ1-S262E/S263E

Plasmid

#141342

Purpose

Lentiviral vector for expression of human NHEJ1-S262E/S263E in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1-S262E/S263E (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Mutation

S262E/S263E

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cn-VA-NHEJ1-S262A/S263A

Plasmid

#141343

Purpose

Lentiviral vector for expression of human NHEJ1-S262A/S263A in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1-S262A/S263A (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Mutation

S262A/S263A

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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