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pAG415Gal_Gcn4 R3W

Plasmid

#232975

Purpose

Galactose iduced expression of Gcn4 R++ W+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 R++ W+

Use

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103R, N126W, D133R, K143R

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 E3HA

Plasmid

#232996

Purpose

Galactose iduced expression of Gcn4 E+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 E+

Use

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D105E, D118E, D139E

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F108A+

Plasmid

#232974

Purpose

Galactose iduced expression of Gcn4 F108A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 F108A

Use

Tags

TEV cleavage site

Expression

Yeast

Mutation

F108A, T121L, A141P

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoAS

Plasmid

#232959

Purpose

Galactose iduced expression of Gcn4 ILVtoAS in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 ILVtoAS

Use

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 S/ArotoL

Plasmid

#231880

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 SArotoL

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 S/ArotoL

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101L, S104L, F108L, Y110L, S117L, S136L, S144L

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol W

Plasmid

#231878

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 solvvol W

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol

Plasmid

#231877

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 solvvol

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 9acidA

Plasmid

#231867

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 9acidA

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 9acidA

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WTSLFD

Plasmid

#231881

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WTSLFD

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 WTSLFD

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F108W, E109T, Y110S, E111L, N112F, L113D

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED W

Plasmid

#231871

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 ILVtoED W

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoAS

Plasmid

#231859

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoAS

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 ILVtoAS

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED

Plasmid

#231857

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 ILVtoED

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123D, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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phU6 NFATC2

Plasmid

#188708

Purpose

sgRNA plasmid encoding hU6 promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pmU6 NFATC2

Plasmid

#188709

Purpose

sgRNA plasmid encoding mU6 promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p7SK NFATC2

Plasmid

#188710

Purpose

sgRNA plasmid encoding 7SK promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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phH1 NFATC2

Plasmid

#188711

Purpose

sgRNA plasmid encoding hH1 promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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sgRNA HBB

Plasmid

#128120

Purpose

Co-expresses sgRNA HBB, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA HBB + GFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA RUNX

Plasmid

#128122

Purpose

Co-expresses sgRNA RUNX, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA RUNX + GFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA HEK

Plasmid

#128123

Purpose

Co-expresses sgRNA HEK, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA HEK + GFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmBI

Plasmid

#126028

Purpose

PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Type

Empty backbone

Use

CRISPR; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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