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Plasmid#162487PurposehyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertEpac-sensor (RAPGEF3 Human, Synthetic)
UseTagsYPet and Turquoise2GLExpressionMammalianMutationPromoterCAGAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6
Plasmid#177849PurposeBacterial Expression of PNRDepositorInsertphotoreceptor cell-specific nuclear receptor (NR2E3 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAKgfp1
Plasmid#14076DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
scFv-SmBiT
Plasmid#192541PurposeExpresses sender cell components of LOTIISDepositorInsertscFv - SmBiT86
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
2x GCN4-LgBiT
Plasmid#192542PurposeExpresses receiver cell components of LOTIISDepositorInsert2x GCN4-LgBiT
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK057
Plasmid#75036PurposeProduces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase, N-terminal His6 (H6EcPurE1) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase (purK Escherichia coli)
UseTagsExpressionBacterialMutationchanges residues 2-7 to His (SSRNNP>HHHHHH)PromoterT7 and n/aAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDnaN-sfGFP
Plasmid#207246PurposeIntegrative plasmid for expressing DnaN-sfGFP in Caulobacter crescentusDepositorInsertDnaN(3' end)-sfGFP
UseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-VC155
Plasmid#22011PurposeMammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assaysDepositorInsertVC155
UseTagsHAExpressionMammalianMutationChanged Alanine 206 to LysinePromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-VN173
Plasmid#22010PurposeMammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assaysDepositorInsertVN173
UseTagsFLAGExpressionMammalianMutationVenus residus 1-172PromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
mCyRFP1-C1
Plasmid#84355PurposeExpresses mCyRFP1 fluorescent protein in an empty backboneDepositorInsertmCyRFP1
UseTagsExpressionMammalianMutationA161K and N41A, reletive to CyRFP1PromoterCMVAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEG03
Plasmid#154138PurposeThis plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_gyrAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise-N1
Plasmid#60559PurposeExpresses the cyan fluorescent protein variant mTurquoiseDepositorInsertmTurquoise
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pxyl-zapT-mScarlet-I
Plasmid#207249PurposeIntegrative plasmid for expressing ZapT-mScarlet-I in Caulobacter crescentus CB15N under Pxyl promoterDepositorInsertZapT-mScarlet-I
UseTagsmScarlet-IExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise-C1
Plasmid#60558PurposeExpresses the cyan fluorescent protein variant mTurquoiseDepositorInsertmTurquoise
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
CyRFP1-C1
Plasmid#84354PurposeExpresses CyRFP1 fluorescent protein in an empty backboneDepositorInsertCyRFP1
UseTagsExpressionMammalianMutationH61M (compared with CyOFP)PromoterCMVAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEG03'
Plasmid#162578PurposeThis plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_gyrAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG01
Plasmid#154136PurposeThis plasmid carries fucO and aldA under the control of P_BAD promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_BADAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG02
Plasmid#154137PurposeThis plasmid carries fucO and aldA under the control of P_thrC3 promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_thrC3Available sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIC-EcTOP
Plasmid#224463PurposeExpression of recombinant Escherichia coli topoisomerase IDepositorInsertEscherichia coli TopA
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIBA2-Int-SpyTag
Plasmid#198038PurposeExpression plasmid for producing SpyTagged stalled secretion variant of intiminDepositorInserteaeA
UseTagsSpyTagExpressionBacterialMutationlacks native signal peptide (amino acids 1-39)PromotertetAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only