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pAAV-synapsin-WHaloCaMP1a

Plasmid

#205308

Purpose

Production of AAV particles encoding WHaloCaMP1a from the synapsin promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-15F11-HA-Halo

Plasmid

#129592

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_FBXO3:1-471

Plasmid

#211071

Purpose

Mammalian expression of full-length human FBXO3. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXO3:M1-F471 (FBXO3 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA5/FRT/TO_TOMM20_HaloTag7

Plasmid

#169330

Purpose

Expression of TOMM20-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag7 (TOMM20 Human)

Use

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-HaloGFP-Na2.4

Plasmid

#228471

Purpose

Mammalian expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

Depositor

Robert Campbell

Article

Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

Insert

HaloGFP-Na2.4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-WHaloCaMP1a-EGFP

Plasmid

#205313

Purpose

Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1_HaloTag9

Plasmid

#175541

Purpose

Lyzosomal expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOMM20_HaloTag9

Plasmid

#169335

Purpose

Expression of HaloTag9 at the mitochondrial surface of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag9 (TOMM20 Human)

Use

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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HaloTag-G protein gamma-2

Plasmid

#67018

Purpose

G protein gamma-2 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-G protein gamma-2 (GNG2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag9_LamB1

Plasmid

#175538

Purpose

Expression of HaloTag9 at the nuclear lamina of the mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9-LamB1

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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HaloTag-G protein beta-1

Plasmid

#67016

Purpose

G protein beta-1 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-G protein beta-1 (GNB1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-RT005-HaloGPM

Plasmid

#163385

Purpose

2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

Halotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).cpSFGFP.HaloTag

Plasmid

#209654

Purpose

Non-responsive control

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.HaloTag

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-HaloTag7-NCOA4

Plasmid

#192806

Purpose

Stable expression of HaloTag7-NCOA4 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-HaloTag7-CHMP4A

Plasmid

#184895

Purpose

Stable expression of HaloTag7-CHMP4A in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

CHMP4A (CHMP4A Human)

Use

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag7_LamB1

Plasmid

#175528

Purpose

Expression of HaloTag7 at the nuclear lamina of the mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag7-LamB1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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ULK1-Halo HRD

Plasmid

#207548

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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UAS-Syt::Halo7

Plasmid

#87648

Purpose

For creating drosophila transgenics expressing UAS-Syt::Halo7

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Syt::Halo7

Use

Tags

Expression

Insect

Mutation

S265G in Syt (please see depositor's commen…

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_RAB7A:1-207

Plasmid

#211096

Purpose

Mammalian expression of full-length human RAB7A. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RAB7A:M1-C207 (RAB7A Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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epB_UbC_CymRV5-nls-Halox2_DEx4

Plasmid

#119907

Purpose

ePiggyBac mammalian expression plasmid for CymR-Halo fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

CymRV5-nls-Halox2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2019

Availability

Academic Institutions and Nonprofits only

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