Addgene

We narrowed to 10,960 results for: Vars;

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pSPL3-ABCC8-27

Plasmid

#135919

Purpose

Encodes ABCC8 exon 27 wild type for the analysis of splicing variants

Depositor

Diana Valverde Pérez

Article

Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

Insert

ATP-binding Cassette subfamily C member 8 (ABCC8 Human)

Use

Exon trapping

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET12a-HAL

Plasmid

#47097

Depositor

Marina Ramirez-Alvarado

Insert

HAL

Use

Tags

ompT

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pET12a-JOH1

Plasmid

#47098

Depositor

Marina Ramirez-Alvarado

Insert

JOH1 (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-DAH

Plasmid

#47096

Depositor

Marina Ramirez-Alvarado

Insert

DAH (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-KIo18/08 C214S

Plasmid

#47099

Depositor

Marina Ramirez-Alvarado

Insert

KIo18/08 C214S (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

C214S

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-103 delProIns

Plasmid

#47095

Depositor

Marina Ramirez-Alvarado

Insert

AL-103 delProIns (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

delete aa95

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
LZRS-Rfa

Plasmid

#31601

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pCAGGS-S-B.1.1.529-FLAG

Plasmid

#180774

Purpose

B.1.1.529 variant S-protein with c-terminal FLAG tag

Depositor

Daniel Conway

Insert

S-protein B.1.1.529

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pRB1080

Plasmid

#71309

Purpose

Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

VQR Cas9 variant

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pRB1083

Plasmid

#71480

Purpose

Expresses VRER Cas9 variant with eef-1A.1 (eft-3) promoter

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

VRER Cas9 variant

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

eft-3 (eef-1A.1)

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV_BiCHATe27_C1V1_eYFP

Plasmid

#213831

Purpose

AAV construct expressing eYFP-tagged chimeric opsin variant C1V1 driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_C1V1_eYFP

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

C1V1-eYFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe10_C1V1_eYFP

Plasmid

#213816

Purpose

AAV construct expressing eYFP-tagged chimeric opsin variant C1V1 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_C1V1_eYFP

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

C1V1-eYFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3_C_VPS35L

Plasmid

#209698

Purpose

Expresses MAC3-tagged VPS35L in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS35L (VPS35L Human)

Use

Tags

MAC3-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_N_VPS26C

Plasmid

#209689

Purpose

Expresses MAC-tagged VPS26C in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS26C (VPS26C Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Use

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pTargeT gE

Plasmid

#60845

Purpose

Expression of the varicella zoster glycoprotein (gE) in mammalian cells

Depositor

Charles Grose

Article

Carpenter et al J Virol. 2011 Sep;85(18):9414-24. doi: 1

Insert

ORF68 (ORF68 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2014

Availability

Academic Institutions and Nonprofits only
pGEL685

Plasmid

#205592

Purpose

NEPE for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

ZmUbi

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBTK622

Plasmid

#180592

Purpose

tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

Depositor

Jeffrey Barrick

Article

Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

Inserts

tdk
kanR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_eGFP

Plasmid

#82714

Purpose

Expresses Myosin VI HMM with a C-terminal eGFP.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

Myosin VI

Use

Tags

FLAG purification tag and eGFP

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-FAK30

Plasmid

#59131

Purpose

Cloning vector for FAK fret sensor with 30 nm helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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