We narrowed to 159,238 results for: addgene

1. pK2-K3

   Plasmid

   #218221

   Purpose

   Fuses Guides 1+2 to guides 3+4 in an of AtU6-promter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK4-J11

   Plasmid

   #218225

   Purpose

   Terminates AtU6-promoter driven sgRNA array with four guides, MoClo Compatible

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK6-J11

   Plasmid

   #218226

   Purpose

   Terminates AtU6-promoter driven sgRNA array with tsix guides, MoClo Compatible

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pK4-K5

   Plasmid

   #218222

   Purpose

   Fuses Guides 3+4 to guides 6+6 in an of AtU6-promter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pK8-J11

   Plasmid

   #218227

   Purpose

   Terminates AtU6-promoter driven sgRNA array with eight guides, MoClo Compatible

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pK6-K7

   Plasmid

   #218223

   Purpose

   Fuses Guides 5+6 to guides 7+8 in an of AtU6-promter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAS-mak10 gRNA

   Plasmid

   #160385

   Purpose

   Expresses guide RNA for targeting MAK10 in yeast

   Depositor

   John McCusker

   Insert

   mak10 gRNA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RNA pol III promoter (tRNA-Tyr)

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV V5-Mospd1

   Plasmid

   #175132

   Purpose

   Lentiviral expression of V5-tagged mouse Mospd1

   Depositor

   Larry Gerace

   Insert

   Mospd1 (Mospd1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV Tyw1-V5

   Plasmid

   #175131

   Purpose

   Lentiviral expression of mouse Tyw1-V5

   Depositor

   Larry Gerace

   Insert

   Tyw1 (Tyw1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPATZ1.1.0-gDNA

    Plasmid

    #132476

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    PATZ1 (PATZ1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZNF449-R.1.0-gDNA

    Plasmid

    #132469

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF449 (ZNF449 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZNF302.1.0-gDNA

    Plasmid

    #132467

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF302 (ZNF302 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZNF253.1.0-gDNA

    Plasmid

    #132461

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF253 (ZNF253 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZFPM2.1.0-gDNA

    Plasmid

    #132459

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZFPM2 (ZFPM2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pZNF3.1.0-gDNA

    Plasmid

    #132440

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF3 (ZNF3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTSHZ1.1.0-gDNA

    Plasmid

    #132434

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    TSHZ1 (TSHZ1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. 4PY6

    Plasmid

    #101272

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    4PY6
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pRSV-Rev

    Plasmid

    #12253

    Purpose

    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Article

    Dull et al J Virol. 1998 Nov . 72(11):8463-71.

    Insert

    Rev (rev )
    

    Use

    Lentiviral; Packaging

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. VV201: ChR2(C128S)-eGFP in fubi

    Plasmid

    #58510

    Purpose

    Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    ChR2(C128S)-eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Cysteine 128 to Serine

    Promoter

    Ubiquitin

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-Vector

    Plasmid

    #214477

    Purpose

    Vector control for mCherry fusions. Created from the backbone of Addgene #55043 mCherry-Ezrin-N-14 from Michael Davidson.

    Depositor

    Natalie Ahn

    Article

    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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