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pKLV2-U6gRNA5(PRDM14_v32_7-4)-PGKpuroBFP-W

Plasmid

#211982

Purpose

Express gRNA against PRDM14 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting PRDM14 (PRDM14 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(PRDM14_v32_7-6)-PGKpuroBFP-W

Plasmid

#211983

Purpose

Express gRNA against PRDM14 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting PRDM14 (PRDM14 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(AAVS1-1)-PGKpuroBFP-W

Plasmid

#211954

Purpose

Express safe-cutter control gRNA with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

safe-cutter control sgRNA_AAVS1-1 (AAVS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(AAVS1-2)-PGKpuroBFP-W

Plasmid

#211955

Purpose

Express safe-cutter control gRNA with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

safe-cutter control sgRNA_AAVS1-2 (AAVS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CTRL00509)-PGKpuroBFP-W

Plasmid

#211956

Purpose

Express non-cutter control gRNA with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

non-cutter control sgRNA_CTRL00509

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CTRL00626)-PGKpuroBFP-W

Plasmid

#211957

Purpose

Express non-cutter control gRNA with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

non-cutter control sgRNA_CTRL00626

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

Plasmid

#189636

Purpose

Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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hTln1-R1R2

Plasmid

#191440

Purpose

Expresses the human TLN1 R1R2 domains in bacteria

Depositor

Ben Goult

Article

Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901

Insert

Human Talin1 R1R2 domains (TLN1 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On shYAP1-6

Plasmid

#193667

Purpose

Tet inducible knockdown of YAP1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On shYAP1-2

Plasmid

#193665

Purpose

Tet inducible knockdown of YAP1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shYAP1-2

Plasmid

#193693

Purpose

Constitutive lentiviral expression of YAP1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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KCC2 scFv [N1/12]

Plasmid

#190520

Purpose

Mammalian Expression of KCC2 scFV. Derived from hybridoma N1/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

KCC2 (Rattus norvegicus) recombinant scFV (Slc12a5 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G6_T804N_Std_Dual_epegRNA_tevopreQ1

Plasmid

#187457

Purpose

Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G6 T804N pegRNA (ATP1A1 Human)

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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Cre-IRES-PuroR

Plasmid

#30205

Purpose

Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

Depositor

Darrell Kotton

Article

Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

Insert

Cre recombinase (cre Enterobacteria phage P1)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 12, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

Plasmid

#204359

Purpose

AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDq-mCherry-P2A-HA-KORD

Use

AAV

Tags

HA (for KORD) and mCherry (for miniDq)

Mutation

For miniDq, the third intracellular loop (ICL3) o…

Promoter

hSyn

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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TRPML1-mCherry

Plasmid

#135189

Purpose

Expresses TRPML1 with C-terminus mCherry tag in mammalian cells.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TRPML1 (Mcoln1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-6xHis [N144/14R]

Plasmid

#177500

Purpose

Mammalian Expression Plasmid of anti-6xHis. Derived from hybridoma N144/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-6xHis recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pINSPECT_SigP:NLuc

Plasmid

#197263

Purpose

Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

Type

Empty backbone

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Expression

Mammalian

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pICOZ-MAG[EF1α-CD19 4-1BB-eGFP]

Plasmid

#218344

Purpose

Donor plasmid of MAG Transposon with CD19 CAR mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pX458-Dual-Guide-Donor-ShuttleOnly

Plasmid

#171098

Purpose

Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Mutation

XbaI site added upstream of U6 promotor. GtoC mu…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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