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Plasmid#214927PurposeExpress miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)DepositorInsertmiRFP670nano-P2A-ERM-gCam6f
UseLentiviralTagsExpressionMammalianMutationPromoterCMVpAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLIC-GST-GINIP
Plasmid#204844PurposeExpress GST tagged-GINIP in bacterial cells for protein purificationDepositorInsertPHF24 (KIAA1045 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC2-gephyrin S268E/270E
Plasmid#199609PurposeEncodes N-terminal eGFP-tagged P1-gephyrin S268/270E for expression in mammalian cells.DepositorInsertGephyrin (Gphn Rat)
UseTagsEGFPExpressionMammalianMutationS268E/S270EPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Lamp1 FLAG
Plasmid#199750PurposeLentiviral construct to stably express Lamp1DepositorInsertLamp1 (Lamp1 Rat)
UseTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc Metap2
Plasmid#199685PurposeTransient expression of Metap2DepositorInsertMETAP2 (METAP2 Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-PKAcat(G1A)-mCherry
Plasmid#181852PurposemCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.DepositorInsertPKAcat-mCherry (PRKACA Human)
UseTagsmCherryExpressionMammalianMutationGlycine residue immediately after start site in P…PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)
Plasmid#185717PurposeAAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoterDepositorInsertsynuclein, alpha (SNCA Human)
UseAAVTagsExpressionMutationChanged Ala 11 to Pro, Val 70 to ProPromoterhSynAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
JAM2_pLENTI-CAG-IRES-GFP
Plasmid#176997PurposeMammalian lentiviral expression vector encoding JAM2DepositorInsertJAM2 (JAM2 Human)
UseLentiviralTagsExpressionMutationPromoterCAGAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRYZL1_pLENTI-CAG-IRES-GFP
Plasmid#177004PurposeMammalian lentiviral expression vector encoding CRYZL1DepositorInsertCRYZL1 (CRYZL1 Human)
UseLentiviralTagsExpressionMutationPromoterCAGAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
DOP1B_pcDNA6.2/EmGFP-Bsd
Plasmid#176979PurposeMammalian expression vector encoding DOP1B and EmGFP-BsdDepositorInsertDOP1B (DOPEY2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
KCNJ15_pcDNA6.2/EmGFP-Bsd
Plasmid#176981PurposeMammalian expression vector encoding KCNJ15 and EmGFP-BsdDepositorInsertKCNJ15 (KCNJ15 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRYZL1_pcDNA6.2/EmGFP-Bsd
Plasmid#176941PurposeMammalian expression vector encoding CRYZL1 and EmGFP-BsdDepositorInsertCRYZL1 (CRYZL1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep
Plasmid#180806PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrepDepositorInsertBTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep (BTLA Human)
UseTagsExpressionBacterialMutationBTLA (Y243F, Y257F, Y282F)PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrep
Plasmid#180807PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrepDepositorInsertBTLAint (AA190-289, Y226F, Y257F, Y282F)-TwinStrep (BTLA Human)
UseTagsExpressionBacterialMutationBTLA (Y226F, Y257F, Y282F)PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y282F)-TwinStrep
Plasmid#180808PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y282F)-TwinStrepDepositorInsertBTLAint (AA190-289, Y226F, Y243F, Y282F)-TwinStrep (BTLA Human)
UseTagsExpressionBacterialMutationBTLA (Y226F, Y243F, Y282F)PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y257F)-TwinStrep
Plasmid#180809PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y257F)-TwinStrepDepositorInsertBTLAint (AA190-289, Y226F, Y243F, Y257F)-TwinStrep (BTLA Human)
UseTagsExpressionBacterialMutationBTLA (Y226F, Y243F, Y257F)PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-2-GST-PD-1icd (AA194-288, Y223F, Y248F)
Plasmid#180815PurposeExpression of GST fused human PD-1 intracellular domain (AA194-288, Y223F, Y248F)DepositorInsertPD-1int (AA194-288, Y223F, Y248F) (PDCD1 Human)
UseTagsExpressionBacterialMutationPD-1 (Y223F, Y248F)PromoterTacAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PWPXLD-EGFR-P667A
Plasmid#133750PurposeEGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmidDepositorInsertMutant P667A EGFR-HA (EGFR Human)
UseLentiviralTagsHA tagExpressionMammalianMutationproline 667 converted to alanine (Please see depo…PromoterEF1-alphaAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR10
Plasmid#167003PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorInsertAR Promoter sgRNA 10 (AR Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v2-10 (-cyt)
Plasmid#137825Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v2-10 (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic region - N213S on the CD44 re…PromoterPGKAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
ST-Tstem(5A)-GFP
Plasmid#162502PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.DepositorUseTagsGFPExpressionMammalianMutationThe mutated Tac stem region is inserted within ST…PromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tac-STC(5A)
Plasmid#162491PurposeExpresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cellsDepositorInsertpartial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
UseTagsGFPExpressionMammalianMutationTac is in partial length with its stem region, TM…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRepair-mTq2-CTNNB1
Plasmid#153433PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmTurquoise2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS(1-720aa)
Plasmid#139687PurposeExpresses N-teminal Flag tagged aa 1- 720 of LARS1DepositorInsertLARS1 (LARS1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2(delta)KL-VSV
Plasmid#139352PurposeExpression of TYK2 protein missing the kinase-like (pseudokinase) domainDepositorInsertTYK2 cDNA deleted of aa594-877 with a Tyr inserted after aa593 (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-deletion of aa 594-877PromoterCMVAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDM304_YFP-IqgC_mRFP-Raf1(RBD)
Plasmid#128698Purposesimultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cellsDepositorInsertsiqgC (full length)
Raf1 kinase (aa 50-132)
UseDictyostelium discoideum expression vectorTagsYFP and mRFPExpressionMutationnonePromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-Lyn-AktAR2-D
Plasmid#125198PurposeSB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporterDepositorInsertLyn-AktAR2-D
UseTransposonTagsLyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]ExpressionMammalianMutationFoxO1 Thr-24 changed to Val rendering this inacti…PromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S159/181/666E
Plasmid#105314PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159/181/666EPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
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