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Showing: 8951 - 9000 of 167150 results

  1. SnFR_V.7.1.2

    Plasmid
    #182809
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Insert
    V.7.1.2-CholineSnFR
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    182809-plasmid-map-sequence-id-358174

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  2. pHH0103 SMURF2 WW domain #3

    Plasmid
    #104226
    Purpose
    Bacterial expression of WW domain #3 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #3 (SMURF2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104226-plasmid-map-sequence-id-204295

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  3. pHH0103 SMURF2 WW domain #2

    Plasmid
    #104225
    Purpose
    Bacterial expression of WW domain #2 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #2 (SMURF2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104225-plasmid-map-sequence-id-204192

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  4. pHH0103 SMURF2 WW domain #1

    Plasmid
    #104224
    Purpose
    Bacterial expression of WW domain #1 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #1 (SMURF2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104224-plasmid-map-sequence-id-201513

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  7. pAAV-synP-FLEX-EGFP-B19G

    Plasmid
    #59333
    Purpose
    Expresses EGFP-P2A-B19G
    Depositor
    Ian Wickersham
    Insert
    EGFP-P2A-B19G
    Use
    AAV and Cre/Lox
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    Synapsin-1
    Available Since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    59333-plasmid-map-sequence-id-92029

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  9. pAW8-aid-V5-eDSR-Turg1:kanMX6

    Plasmid
    #169365
    Purpose
    C-terminal aid-V5-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    1XV5
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169365-plasmid-map-sequence-id-333205

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  10. pAW8-aid-5Myc-Turg1:kanMX6

    Plasmid
    #169356
    Purpose
    C-terminal aid-5Myc degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    5XMyc
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169356-plasmid-map-sequence-id-333204

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  11. R777-E191 Hs.PRKAA1

    Plasmid
    #70475
    Purpose
    Gateway ORF clone of human PRKAA1 [NM_006251.5] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PRKAA1 (PRKAA1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70475-plasmid-map-sequence-id-125924

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  12. PP-RAB11A

    Plasmid
    #25120
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF13_0119:C34946
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. Nup133_mEGFP(-9a)

    Plasmid
    #163419
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)
    Depositor
    Sanford Simon
    Insert
    Nup133 (NUP133 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163419-plasmid-map-sequence-id-321638

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  14. pLenti-CMV-GFP-miRE_shRen-Blast

    Plasmid
    #135684
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Available Since
    March 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135684-plasmid-map-sequence-id-327610

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  15. pLenti-CMV-tdTomato-miRE_shRen-Blast

    Plasmid
    #135686
    Purpose
    Constitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135686-plasmid-map-sequence-id-327617

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  16. mOrange2-CytERM-N-17

    Plasmid
    #57952
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 549, Emission: 565
    Depositor
    Michael Davidson
    Insert
    CytERM (AA1-29)
    Tags
    mOrange2
    Expression
    Mammalian
    Mutation
    Only first 29 AA of CytERM with mutations V8G, H2…
    Promoter
    CMV
    Available Since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    57952-plasmid-map-sequence-id-215282

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  17. pEGB3_alpha1 Pnos:P19:Tnos (GB2762)

    Plasmid
    #160632
    Purpose
    TU for expression of P19 driven by Nos promoter.
    Depositor
    Diego Orzaez
    Insert
    P19
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos
    Available Since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160632-plasmid-map-sequence-id-321966

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  19. UC20m

    Plasmid
    #121040
    Purpose
    MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).
    Depositor
    Richard Murray
    Insert
    gN2 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121040-plasmid-map-sequence-id-236353

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  20. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

    Plasmid
    #137859
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137859-plasmid-map-sequence-id-274442

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  21. R777-E133 Hs.MLST8

    Plasmid
    #70417
    Purpose
    Gateway ORF clone of human MLST8 [NM_022372.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    MLST8 (MLST8 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70417-plasmid-map-sequence-id-125866

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  24. pE-SUMO CRYGB

    Plasmid
    #80751
    Purpose
    Expression of human gamma B-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGB (CRYGB Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80751-plasmid-map-sequence-id-153519

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  25. pSpy

    Plasmid
    #29588
    Depositor
    Marc Fiedler
    Insert
    Spy (spy E.coli BL21(DE3))
    Expression
    Bacterial
    Mutation
    processed form of Spy starting at residue 24 (Rem…
    Available Since
    April 28, 2011
    Availability
    Academic Institutions and Nonprofits only

  26. L4752

    Plasmid
    #1675
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, pes10UAS-270, pes10Tsa, T7p, gfpN, IVSR, gf…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1675-plasmid-map-sequence-id-285

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  27. Mouse NCKX2-AS-long (pcDNA3.1+)

    Plasmid
    #75199
    Purpose
    Mammalian expression of SLC24A2
    Depositor
    Jonathan Lytton
    Insert
    SLC24A2 (Slc24a2 Mouse)
    Expression
    Mammalian
    Mutation
    alternatively spliced exon present
    Promoter
    CMV
    Available Since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    75199-plasmid-map-sequence-id-142841

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  28. mEos-A69V-Paxillin-22

    Plasmid
    #57338
    Purpose
    Localization: Focal Contacts, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Paxillin
    Tags
    mEos A69V
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. T12m

    Plasmid
    #121031
    Purpose
    MoClo golden gate assembly DE part ECK120029600 (378x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120029600 (strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121031-plasmid-map-sequence-id-249041

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  30. MTMR6A

    Plasmid
    #42483
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MTMR6A
    Tags
    His6
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. mApple-Moesin-C-14

    Plasmid
    #54927
    Purpose
    Localization: Cytoskeleton/Membrane, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Moesin (MSN Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  32. R777-E251 Hs.RASSF1

    Plasmid
    #70535
    Purpose
    Gateway ORF clone of human RASSF1 [NM_007182.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASSF1 (RASSF1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70535-plasmid-map-sequence-id-125984

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  33. mPA-GFP-C-Src-7

    Plasmid
    #57127
    Purpose
    Localization: Membrane/Vesicles, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    C-Src
    Tags
    mPA-GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. INPP5EA

    Plasmid
    #42481
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    INPP5EA
    Tags
    His6
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. PS-CFP2-MCPA2-N-18

    Plasmid
    #57235
    Purpose
    Localization: Actin Capping Protein , Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Insert
    MCPA2
    Tags
    PS-CFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 13, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    57235-plasmid-map-sequence-id-221545

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  36. R777-E196 Hs.PRKAB1-nostop

    Plasmid
    #70480
    Purpose
    Gateway ORF clone of human PRKAB1 [NM_006253.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PRKAB1 (PRKAB1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70480-plasmid-map-sequence-id-125929

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  37. mKO2-Peroxisomes-2

    Plasmid
    #54769
    Purpose
    Localization: Peroxisomes, Excitation: 551, Emission: 565
    Depositor
    Michael Davidson
    Insert
    mKO2-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. R777-E266 Hs.RASSF7-nostop

    Plasmid
    #70550
    Purpose
    Gateway ORF clone of human RASSF7 [NM_001143993.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASSF7 (RASSF7 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70550-plasmid-map-sequence-id-358342

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  39. L4664

    Plasmid
    #1666
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo3-2341, myo3Tsa, IVSA, pat3MbLS, gfpN, IVSR, g…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1666-plasmid-map-sequence-id-276

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  40. R777-E304 Hs.SAV1-nostop

    Plasmid
    #70588
    Purpose
    Gateway ORF clone of human SAV1 [NM_021818.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SAV1 (SAV1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70588-plasmid-map-sequence-id-126037

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  41. R777-E188 Hs.PPP1CA-nostop

    Plasmid
    #70472
    Purpose
    Gateway ORF clone of human PPP1CA [NM_002708.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PPP1CA (PPP1CA Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70472-plasmid-map-sequence-id-125921

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  42. pCB94

    Plasmid
    #69743
    Purpose
    Retroviral expression of PP2A beta
    Depositor
    Iain Cheeseman
    Insert
    PP2A beta (PTPA Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Available Since
    Dec. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  43. SP65U1-301a

    Plasmid
    #105821
    Purpose
    plasmid for the overexpression of miR-301a under the U1 expression cassette inserted in the pSP65 plasmid
    Depositor
    Cecilia Mannironi
    Insert
    MIRNA301 (Mir301 Mouse)
    Expression
    Mammalian
    Promoter
    U1
    Available Since
    Jan. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    105821-plasmid-map-sequence-id-202521

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  44. 4I79

    Plasmid
    #51319
    Depositor
    Cheryl Arrowsmith
    Insert
    4I79 (NUP43 Human)
    Use
    Baculovirus expression
    Tags
    His
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  45. pJK365

    Plasmid
    #72404
    Purpose
    Produces Acetobacter aceti 1023 Est01
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioester hydrolase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72404-plasmid-map-sequence-id-134631

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  46. mEos-A69V-N1

    Plasmid
    #54754
    Purpose
    Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mEos A69V, V123T, T158H
    Expression
    Mammalian
    Available Since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. pHH0103 WBP4 WW domain #2

    Plasmid
    #104253
    Purpose
    Bacterial expression of WW domain #2 from WBP4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WBP4 WW domain #2 (WBP4 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104253-plasmid-map-sequence-id-204383

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  48. (P79) OCN/Wnt 10b transgene

    Plasmid
    #25878
    Depositor
    Ormond MacDougald
    Insert
    Wnt10b (Wnt10b Mouse)
    Use
    Mouse Targeting
    Tags
    B-globin intron/poly A coding sequence and OCN pr…
    Expression
    Mammalian
    Available Since
    Oct. 21, 2010
    Availability
    Academic Institutions and Nonprofits only

  49. pE-SUMO CRYGC

    Plasmid
    #80754
    Purpose
    Expression of human gamma C-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGC (CRYGC Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80754-plasmid-map-sequence-id-153567

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  50. PP-CyP3

    Plasmid
    #25108
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd2_1660:C33295
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 8951 - 9000 of 167150 results