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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,439 results for: eng

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Showing: 3581 - 3600 of 14439 results

  1. 868 pSG5L HA NLS PTEN G129R

    Plasmid
    #10935
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    G129R
    Available since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  3. pTXB1-TP3

    Plasmid
    #160086
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA
    Depositor
    Pier Pandolfi
    Publication
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160086-plasmid-map-sequence-id-313733

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  10. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid
    #181792
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181792-plasmid-map-sequence-id-358226

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  11. pYL-kasOp-FnCas12a3

    Plasmid
    #177875
    Purpose
    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.
    Depositor
    Yunzi Luo
    Publication
    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptomyces codon optimized the FnCas12a mutant EP16
    Use
    CRISPR
    Promoter
    kasOp*
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    177875-plasmid-map-sequence-id-352013

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  12. pNOC_hfnCas12a_CRATES

    Plasmid
    #176251
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitpl
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176251-plasmid-map-sequence-id-347017

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  17. LP538

    Plasmid
    #90202
    Purpose
    pCMV-myc with cDNA encoding HsTRAPPC8 full length
    Depositor
    Lotte Pedersen
    Publication
    Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRAPPC8 (TRAPPC8 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    full length protein, no mutations
    Promoter
    CMV
    Available since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    90202-plasmid-map-sequence-id-178310

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  19. PX377 Corynebacter diphtheriae Cas9

    Plasmid
    #68314
    Purpose
    Corynebacter diphtheriae Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    68314-plasmid-map-sequence-id-119522

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  20. PX396 Parvibaculum lavamentivorans Cas9

    Plasmid
    #68333
    Purpose
    Parvibaculum lavamentivorans Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PlCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68333-plasmid-map-sequence-id-119549

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Showing: 3581 - 3600 of 14439 results