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WT C-His Ana GvpC in pET28a

Plasmid

#85732

Purpose

Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

Added an extra glycine after the methionine at th…

Promoter

T7

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCS_FLPER(T2)

Plasmid

#31303

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

FLP-ER ligand binding domain (ESR1 Budding Yeast, Human)

Expression

Mammalian

Mutation

full length FLP linked to ligand binding domain o…

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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813 pSG5L HA PTEN 1-274

Plasmid

#10740

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

Contains PTEN amino acids 1-274.

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pBAD25

Plasmid

#231547

Purpose

L-arabinose inducible expression of heterologous genes, pBAD24-based backbone with KanR selective marker

Depositor

Martin Wiedmann

Article

Schumann et al J Microbiol Methods. 2025 Mar 18;232-234

Type

Empty backbone

Expression

Bacterial

Promoter

pBAD

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

Plasmid

#197028

Purpose

AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6

Depositor

Xiangfeng Kong

Article

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Insert

humanized enOsCas12f1

Use

AAV

Mutation

D52R / T132R

Promoter

MHCK7, hU6

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159814

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-Aurora

Plasmid

#98167

Purpose

red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Aurora gene

Tags

mCherry

Expression

Mammalian

Mutation

V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

Promoter

CMV (+enhancer)

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-GFP-PTEN-G129E

Plasmid

#30544

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

GFP-PTEN-G129E (PTEN Human)

Expression

Mammalian

Mutation

GFP-PTEN-G129E

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pTH733-2µ-RLuc/maxCFLuc

Plasmid

#40608

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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cpLOV2-V7

Plasmid

#168993

Purpose

circularly permuted LOV2 variant-V7

Depositor

Yubin Zhou

Article

He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

Insert

cpLOV2-V7

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->GFPlig

Plasmid

#162223

Purpose

Lentiviral vector for constitutive expression of GFP ligand in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGS-CD64-2

Plasmid

#109191

Purpose

Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

Full-length engineered CD64, derived from human CD64

Tags

Twin-Strep

Expression

Insect

Mutation

engineered for efficient expression, see publicat…

Promoter

Polyhedrin

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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(114) pcDNA3.1-GFP-Flag-JunB-EPEA

Plasmid

#160743

Purpose

Expresses a GFP tagged JunB with Flag and EPEA tags.

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

GFP-Flag-JunB-EPEA (JUN Human)

Tags

EPEA, Flag, and GFP

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-CCR5

Plasmid

#98950

Purpose

Mammalian expression plasmid for N-terminal HA-tagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Tags

HA (YPYDVPDYA) epitope tag and Signal/leader sequ…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

Plasmid

#91880

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pUNIV-SIG-5HTR4D100A

Plasmid

#16312

Depositor

Bruce Conklin

Article

Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

Insert

h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (HTR4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Added FLAG tag; Aspartic Acid 100 changed to Alan…

Available Since

Jan. 3, 2008

Availability

Academic Institutions and Nonprofits only
pTFUbiCre

Plasmid

#139670

Purpose

Expression cassette for Cre recombinase in T-DNA vector

Depositor

James Birchler

Article

Gaeta et al Chromosoma. 2013 Jun;122(3):221-32. doi:

Insert

Cre recombinase

Expression

Plant

Promoter

Maize Ubiquitin

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pRG216

Plasmid

#64528

Purpose

Centromeric E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pRG201

Plasmid

#64394

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pXP622

Plasmid

#26849

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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