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Flag pLJM1 RagD 121L

Plasmid

#19318

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Q121L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pCB6-HFE-EGFP

Plasmid

#12104

Depositor

Pamela Bjorkman

Article

Giannetti et al J Biol Chem. 2004 Jun 11. 279(24):25866-

Insert

HFE (HFE Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pQdCas9-sgempty

Plasmid

#159523

Purpose

Expression of dCas9 driven by lux cassette from Vibrio fischeri

Depositor

Radhakrishnan Mahadevan

Article

Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

Insert

quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-dfROX

Plasmid

#137170

Purpose

Lentiviral transfer vector for expression of roGFP2 and HyPer Red

Depositor

Shaun McCullough

Article

Faber et al Toxicol Sci. 2020 Jun 11. pii: 5856111.

Insert

roGFP2-2A-HyPer Red

Use

Lentiviral

Promoter

EF1a

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NKX3.1

Plasmid

#42897

Depositor

Edward Gelmann

Article

Steadman et al Nucleic Acids Res. 2000 Jun 15;28(12):23

Insert

NKX3.1 (NKX3-1 Human)

Expression

Mammalian

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only
hRIP3 GFP RHIM mut

Plasmid

#41385

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

RHIM mutant (AAAA-459-462)

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only
pCDB179-BhrPETase

Plasmid

#218692

Purpose

Encodes codon optimized BhrPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

BhrPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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AAV.hSyn.GLPLight-ctr

Plasmid

#187468

Purpose

Expresses GLPLight-ctr in mammalian cells

Depositor

Tommaso Patriarchi

Article

Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

Insert

GLPLight-ctr

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AcrIIA2

Plasmid

#101042

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA2

Tags

GST

Expression

Bacterial

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAd-PV-NES-DsRed

Plasmid

#17286

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

PV-NES (Pvalb Rat)

Use

Adenoviral

Tags

DSR and Myc

Expression

Mammalian

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only
Mito-Lapronic/pGW1

Plasmid

#140754

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Mito-Lapronic

Tags

Mitochondrial Sequence Signal

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCiVSP(C363S)-SD64TF

Plasmid

#80334

Purpose

Expresses Ciona VSP (without phosphatase activity) in Xenopus Oocyte

Depositor

Yasushi Okamura

Article

Murata et al Nature. 2005 Jun 30;435(7046):1239-43. E

Insert

Ciona intestinalis voltage-sensing phosphatase (C363S) (vsp Ciona intestinalis)

Use

Xenopus oocyte expression

Mutation

Changed Cysteine 363 to Serine

Promoter

SP6

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Vimentin/pcDNA3

Plasmid

#67772

Purpose

Vimentin-targeted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor-Vimentin

Tags

Vimentin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-HA GST RagA 21L

Plasmid

#19299

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Tags

GST and HA

Expression

Mammalian

Mutation

T21L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
His6-PRC1

Plasmid

#69111

Purpose

For bacterial expression of recombinant human PRC1 tagged with His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Protein Regulator of Cytokinesis 1 (PRC1 Human)

Tags

His6

Expression

Bacterial

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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myc-hULK2

Plasmid

#31966

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK2 (ULK2 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
(R42A) Cyclin B1-GFP (62)

Plasmid

#61849

Purpose

mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

Depositor

Jonathon Pines

Article

Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

Insert

cyclin B1 (CCNB1 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

V5A R42A

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDHt-SK

Plasmid

#63615

Purpose

The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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Erk2

Plasmid

#29582

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Erk2 (Mapk1 Rat)

Tags

HIS6 and TEV

Expression

Bacterial

Mutation

Starting Met removed from Erk2

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only

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TRPML1-CFP

Plasmid

#18827

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Tags

CFP

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only