Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,939 results for: gateway

Showing: 9001 - 9050 of 18939 results

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pSLIK_hygro_3xflag_NRF2(RR)

Plasmid

#136535

Purpose

Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

3x FLAG

Expression

Mammalian

Mutation

This plasmid is developed by mutating 3 base pair…

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-SPHK1

Plasmid

#23704

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SPHK1 (SPHK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PDK4

Plasmid

#23577

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDK4 (PDK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SNX16

Plasmid

#23617

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SNX16 (SNX16 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-SLC32A1_STOP

Plasmid

#161278

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC32A1 (SLC32A1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-SRPK1

Plasmid

#23582

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SRPK1 (SRPK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-SLC6A1_STOP

Plasmid

#161408

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A1 (SLC6A1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223-PAK4

Plasmid

#23713

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK4 (PAK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ULK3

Plasmid

#23646

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ULK3 (ULK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PANK4

Plasmid

#23419

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PANK4 (PANK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR207 SARS-CoV-2 NSP1 Delta RC

Plasmid

#164522

Purpose

Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Insert

NSP1 Delta RC (ORF1ab )

Expression

Mammalian

Mutation

Insert of NSP1 gene's CDS from SARS-CoV-2 is…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-OXSR1

Plasmid

#23847

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

OXSR1 (OXSR1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NAGK

Plasmid

#23785

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NAGK (NAGK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-SLC52A3_STOP

Plasmid

#161347

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC52A3 (C20orf54 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-NLK

Plasmid

#23642

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NLK (NLK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PKDCC

Plasmid

#23387

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PKDCC (PKDCC Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-LETMD1

Plasmid

#132294

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

LETMD1 (LETMD1 Human)

Expression

Mammalian

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-TIE1

Plasmid

#23946

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TIE1 (TIE1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TEC

Plasmid

#23885

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TEC (TEC Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PIM2

Plasmid

#23587

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIM2 (PIM2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TNNI3K

Plasmid

#23469

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TNNI3K (TNNI3K Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PRKY

Plasmid

#23506

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKY (PRKY Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-VRK2

Plasmid

#23534

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

VRK2 (VRK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-LETMD1_STOP

Plasmid

#161460

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

LETMD1 (LETMD1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221-ANKH_STOP

Plasmid

#161444

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

ANKH (ANKH Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223-YSK4

Plasmid

#23616

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

YSK4 (YSK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-VRK3

Plasmid

#23687

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

VRK3 (VRK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-RBKS

Plasmid

#23681

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RBKS (RBKS Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MYO3B

Plasmid

#23524

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MYO3B (MYO3B Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TESK1

Plasmid

#23830

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TESK1 (TESK1 Human)

Use

Gateway Cloning

Mutation

E4G

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TESK2

Plasmid

#23695

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TESK2 (TESK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR207 SARS-CoV-2 NSP1 124A/125A

Plasmid

#164523

Purpose

Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions R124A/K125A)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Insert

NSP1 124A/125A (ORF1ab )

Expression

Mammalian

Mutation

Insert of NSP1 gene's CDS from SARS-CoV-2 is…

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-STK33

Plasmid

#23823

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK33 (STK33 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pENTR1A_mmFbxw7a (closed)

Plasmid

#160101

Purpose

Expresses murine Fbxw7 alpha in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Gateway Cloning

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PI4KAP2

Plasmid

#23601

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PI4KAP2 (PI4KAP2 Human)

Use

Gateway Cloning

Mutation

V154G

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-ACE2 receptor 19-615

Plasmid

#167012

Purpose

Gateway-compatible Entry vector encoding ACE2 receptor (aa19-615) interacting with spike (S1) RBD domain from SARS-CoV-2

Depositor

Spyros Petrakis

Article

Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

Insert

ACE2 (ACE2 Human)

Expression

Mammalian

Mutation

Amino acids 19-615, No stop codon

Available Since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

Plasmid

#156405

Purpose

Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4A-WT (KRAS Human)

Use

Gateway Cloning

Tags

mScarlet-I

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-FLJ40852

Plasmid

#23377

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLJ40852 (FLJ40852 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PHKB

Plasmid

#23859

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PHKB (PHKB Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-XYLB

Plasmid

#23639

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

XYLB (XYLB Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MGC42105

Plasmid

#23596

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MGC42105 (NIM1 Human)

Use

Gateway Cloning

Mutation

Y5F

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PION

Plasmid

#23369

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PION (PION Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-BMP2KL

Plasmid

#23399

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BMP2KL (BMP2KL Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLV-hPGK-DEST-IRES-Puro

Plasmid

#253773

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a hPGK promoter for expression of a gene of interest linked to puromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1alpha-DEST-IRES-Puro

Plasmid

#253769

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to puromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-UbC-DEST-IRES-Puro

Plasmid

#253777

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to puromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-UbC-DEST-IRES-Neo

Plasmid

#253776

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to neomycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-UbC-DEST-IRES-EGFP

Plasmid

#253778

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to EGFP by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1alpha-DEST-IRES-EGFP

Plasmid

#253770

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to EGFP by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-TRE-DEST-UbC-tTSrtTA-IRES-EGFP

Plasmid

#253782

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a Tet-On inducible promoter for expression of a gene of interest linked to EGFP by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge

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