We narrowed to 18,451 results for: Met

1. pU6_(GLuc)_INT[P4-P6(3xPP7)]

   Plasmid

   #68432

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CLPIT Cry2PHR-mCh-Rac1

   Plasmid

   #42955

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-Rac1 (RAC1 Mustard Weed, Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   Tet-Off promoter

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFAB822

   Plasmid

   #47856

   Purpose

   BIOFAB reporter plasmid for measuring BBa_B1006 termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   BBa_B1006 terminator
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLTetO11

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFAB803

   Plasmid

   #47847

   Purpose

   BIOFAB reporter plasmid for measuring T7 early termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   T7 early terminator
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLTetO2

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaG-24

   Plasmid

   #172750

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-24
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21-pelB-LaG-9

   Plasmid

   #172742

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-9
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

   Plasmid

   #69227

   Purpose

   Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS4 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCardinal-CytERM-N-17

   Plasmid

   #56158

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   CytERM
   

   Tags

   mCardinal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pcDNA3_HLA-cmyc-GRM3 C127A

   Plasmid

   #140451

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaG-8

    Plasmid

    #172761

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-8
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21-pelB-LaG-5

    Plasmid

    #172760

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-5
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Clover-Zyxin-6

    Plasmid

    #56531

    Purpose

    Localization: Focal Adhesions, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Zyxin (ZYX Human)
    

    Tags

    Clover

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. sspa-GCaMP6f

    Plasmid

    #67561

    Purpose

    short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    sspa-GCaMP6f
    

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pRSFDuet-1_VNp-DARPinOFF7

    Plasmid

    #182397

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-DARPinOFF7
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. POT10-RFP

    Plasmid

    #65326

    Purpose

    receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. POT8-RFP

    Plasmid

    #65324

    Purpose

    receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. XW20

    Plasmid

    #65836

    Purpose

    Punc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    OR74A quinic acid utilization activator (qa-1F)
    

    Expression

    Worm

    Mutation

    The endogenous SphI, KpnI and SacI sites in QF ar…

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. POT7-RFP

    Plasmid

    #65323

    Purpose

    receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. POT6-RFP

    Plasmid

    #65322

    Purpose

    receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. M-ST1-VPR

    Plasmid

    #68498

    Purpose

    nuclease competent ST1-Cas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    ST-Cas9-VPR
    

    Tags

    VPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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