We narrowed to 28,135 results for: Unk

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pVenus-N1 cyclin B1 L45A (1324)

Plasmid

#61851

Purpose

mammalian expression of cyclin B1 L45 A mutant fused to Venus

Depositor

Jonathon Pines

Article

Matsusaka et al Biol Open. 2014 Sep 12;3(10):904-12. doi

Insert

cyclin B1 (CCNB1 Human)

Tags

Venus

Expression

Mammalian

Mutation

L45A

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
pKV006

Plasmid

#64764

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable V5 tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pHL718

Plasmid

#62826

Purpose

Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

gfp
mCherry

Use

Synthetic Biology

Tags

ipex leader and ompC leader

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL1855

Plasmid

#53041

Purpose

PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrA

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
tetR
csrA

Use

Synthetic Biology

Tags

LVA, RBS(st3), and glgC leader

Expression

Bacterial

Promoter

pConNoHind and pLlac0-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pPSmF

Plasmid

#226912

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in forward orientation

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pPSmR

Plasmid

#226913

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in reverse orientation

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1:ChrA-C63S-C66S

Plasmid

#225082

Purpose

Expresses precursor peptide ChrA-C63S/C66S in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA-C63S/C66S

Tags

His6

Expression

Bacterial

Mutation

C63S/C66S

Promoter

T7

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1:ChrA-C66S

Plasmid

#225081

Purpose

Expresses precursor peptide ChrA-C66S in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA-C66S

Tags

His6

Expression

Bacterial

Mutation

C66S

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1:ChrA-C63S

Plasmid

#225080

Purpose

Expresses precursor peptide ChrA-C63S in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA-C63S

Tags

His6

Expression

Bacterial

Mutation

C63S

Promoter

T7

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1:ChrH-ChrI

Plasmid

#225083

Purpose

Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH and partner protein ChrI in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrH

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1302-AAV-EFSNC-dCjCas9-KRAB

Plasmid

#223147

Purpose

Expression of KRAB with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1312-AAV-EFSNC-dSaCas9-KRAB

Plasmid

#223157

Purpose

Expression of KRAB with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSD118A

Plasmid

#218140

Purpose

Expresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coli

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD0_ZF2-3

Tags

AviTag-6xHis-MBP-TEV-(GGGGS)2

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD068F

Plasmid

#218137

Purpose

Lentiviral vector expressing evolved degron SD36 fused to eGFP with mCherry control.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD36-eGFP-IRES2-mCherry

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD113A

Plasmid

#218133

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved degron SD56

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD56

Tags

rpoZ

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJM154

Plasmid

#218121

Purpose

Hybrid circuit displaying FKBP12 bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

FKBP12
luxAB

Use

Luciferase

Tags

434cI_RR69

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF_641-1574_AH

Plasmid

#148936

Purpose

Insect Expression of DmGIGYF1_641-1574

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF1_641-1574 (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-F361AF367A_AH

Plasmid

#148937

Purpose

Insect Expression of DmGIGYF-F361AF367A

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-F361AF367A (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-W349A_AH

Plasmid

#148938

Purpose

Insect Expression of DmGIGYF-W349A

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-W349A (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-WFFtoAAA_AH

Plasmid

#148939

Purpose

Insect Expression of DmGIGYF-WFFtoAAA

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-WFFtoAAA (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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