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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,807 results for: golden gate

Showing: 3601 - 3620 of 3807 results

  1. pGGC_RT_XVE

    Plasmid
    #136970
    Purpose
    Goldengate/Greengate module for generation of beta-estradiole inducible genes.
    Depositor
    Klaus Theres
    Insert
    XVE
    Expression
    Bacterial
    Mutation
    BsaI site domestication 834 T > A,
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136970-plasmid-map-sequence-id-270565

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  2. pGGA_RT_Sa_Cas9

    Plasmid
    #136962
    Purpose
    Goldengate/Greengate module for genome editing, using the SaCAS9.
    Depositor
    Klaus Theres
    Insert
    SaCAS9
    Use
    CRISPR
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    136962-plasmid-map-sequence-id-272380

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  3. pGGD_RT_biChromium

    Plasmid
    #136974
    Purpose
    Goldengate/Greengate C-tag module, containing a dual fluorescent marker based on the P2A peptide
    Depositor
    Klaus Theres
    Insert
    P2A sequence
    Expression
    Bacterial
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136974-plasmid-map-sequence-id-270595

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  4. pGGF_RT_FAST_Tq2CFP

    Plasmid
    #136981
    Purpose
    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence
    Depositor
    Klaus Theres
    Insert
    seed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
    Expression
    Bacterial
    Mutation
    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…
    Available Since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136981-plasmid-map-sequence-id-269096

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  5. pGGF_RT_FAST_Venus

    Plasmid
    #136980
    Purpose
    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence
    Depositor
    Klaus Theres
    Insert
    seed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
    Expression
    Bacterial
    Mutation
    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…
    Available Since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136980-plasmid-map-sequence-id-269080

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  8. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  11. pAGT9054

    Plasmid
    #219430
    Purpose
    Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS
    Depositor
    Tom Schreiber
    Article
    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000
    Insert
    LB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
    Use
    CRISPR; Moclo compatible level 1 module (position…
    Tags
    PapE-4LF2
    Expression
    Plant
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219430-plasmid-map-sequence-id-441037

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  12. pAGT9569

    Plasmid
    #219431
    Purpose
    Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS
    Depositor
    Tom Schreiber
    Article
    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000
    Insert
    LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
    Use
    CRISPR and Synthetic Biology; Moclo compatible le…
    Tags
    PapE-4LF2
    Expression
    Plant
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    219431-plasmid-map-sequence-id-436703

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  19. pAAV-ITR-CMV-mCherry-1xU6-LeuIGI2

    Plasmid
    #217368
    Purpose
    Expresses E. coli leucine tRNA variant "LeuIGI2" and a wild-type mCherry reporter; can be packaged into AAV
    Depositor
    Abhishek Chatterjee
    Article
    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2
    Insert
    E. coli leucine tRNA mutant, "LeuIGI2" for TAG suppression
    Use
    AAV
    Expression
    Mammalian
    Mutation
    C2G, C3G, G6U, A7G, U77C, C78G, G81C, G82C, U83C
    Promoter
    U6
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    217368-plasmid-map-sequence-id-430295

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Showing: 3601 - 3620 of 3807 results