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LentiCRISPR v2-sgLKB1 clone1

Plasmid

#162125

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos

Plasmid

#211842

Purpose

Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Inserts

LRR-NEL
GAL4-VP16-GR

Use

Synthetic Biology

Tags

HA, Myc, and mCherry

Expression

Plant

Mutation

Promoter

6xUAS-35Smini and pUBQ10

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMito-miRFP718nano

Plasmid

#195754

Purpose

Mitochondria labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

Human cytochrome C oxidase subunit VIII-miRFP718nano

Use

Tags

miRFP718nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-PGK-Puro

Plasmid

#227269

Purpose

Empty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Zeo

Plasmid

#227268

Purpose

Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_N_ELF2

Plasmid

#187737

Purpose

MAC-tagged gene expression of human ELF2

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ELF2 (ELF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG LAP2

Plasmid

#15738

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-KLF5

Plasmid

#40918

Depositor

Jin-Tang Dong

Article

Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

Insert

Kruppel-like factor 5 (KLF5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pHR V5-RNF25 dRING_IRES-mCherry

Plasmid

#198387

Purpose

RNF25 (?RING) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF25 (RNF25 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

RING deletion (135-201)

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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PCS4 3XFLAG-PPARgamma1

Plasmid

#78769

Purpose

To overexpress PPARgamma1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_1 (Pparg Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro LAP2

Plasmid

#15740

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
LentiCRISPR v2-sgLKB1 clone3

Plasmid

#162127

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pME18s-HA-hFIP200

Plasmid

#24303

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

FIP200 (RB1CC1 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pCRISPRi

Plasmid

#199808

Purpose

vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

Depositor

Steve Salipante

Article

Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

Inserts

dCas9
sgRNA with GFP cassette

Use

CRISPR

Tags

Expression

Mutation

Promoter

P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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Wild-type AdPol vector

Plasmid

#131685

Purpose

A lentiviral vector that constitutively expresses the wild-type adenovirus DNA polymerase

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus DNA polymerase

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

June 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-STAG2-wildtype

Plasmid

#31972

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pTW072M

Plasmid

#115960

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl2 (nsP2 Q739L)

Use

Synthetic Biology

Tags

Expression

Mutation

nsP2 Q739L

Promoter

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag-RARalpha

Plasmid

#35555

Depositor

Harish Srinivas, Jonathan Kurie

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Use

Tags

Flag and His

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-IP HA-Parkin

Plasmid

#38248

Depositor

Noboru Mizushima

Article

Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

Insert

Parkin (PRKN Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA cyclin D1 HA

Plasmid

#11181

Depositor

Bruce Zetter

Article

Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

Insert

cyclin D1 (CCND1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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