We narrowed to 70,738 results for: LAS

1. gWiz_BM40-vMAT2-VH-hIgG1-CH1-TS-HIS

   Plasmid

   #218442

   Purpose

   mammalian expression plasmid for the heavy chain fragment of an anti-vMAT2 Fab domain

   Depositor

   Axel Brunger

   Insert

   vMAT2-Vh-hIgG1-CH4
   

   Use

   Affinity Reagent/ Antibody

   Tags

   BM40 and TwinStrep-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAM/CBA-eGFP-miRNegativex3-WPRE-bGHpA

   Plasmid

   #194249

   Purpose

   Expresses 3 non-targeting miRNAs

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   miRNegative
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV/CBA::EGFP-P2A-tC3

   Plasmid

   #129476

   Purpose

   AAV2 transfer plasmid for truncated C3 with EGFP under control of the CBA promoter

   Depositor

   Robert Gross

   Insert

   truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBA

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENN.AAV.SV40-si-MAPRE1

   Plasmid

   #187862

   Purpose

   a human siRNA-expressing plasmid targeting MAPRE1

   Depositor

   Yan Zeng

   Insert

   si-MAPRE1 (MAPRE1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKIIa-hM4D(Gi)-mCherry-WPRE-pA(Short)

   Plasmid

   #183704

   Purpose

   AAV to express hM4D(Gi)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit

   Depositor

   Jonathan Ploski

   Insert

   hM4D(Gi)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CaMKIIa-hM3D(Gq)-mCherry-WPRE-pA(Short)

   Plasmid

   #183705

   Purpose

   AAV to express hM3D(Gq)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit

   Depositor

   Jonathan Ploski

   Insert

   hM3D(Gq)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCellFree_KA1_SARS-COV-2_ORF6

   Plasmid

   #169392

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus ORF6 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _ORF6 (ORF6 SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Q-JMJD2DA-4

   Plasmid

   #166553

   Purpose

   scFv of a human scaffold targeting Lysine-specific demethylase 4D. Antigen coverage aa 1-342 of 523

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBbi-Pur-LPAR

   Plasmid

   #125205

   Purpose

   SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide

   Depositor

   Eric Davis

   Insert

   Lyn-Phospho-Activity-Reporter, Empty
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIC232

    Plasmid

    #69737

    Purpose

    Retroviral expression of Bod1

    Depositor

    Iain Cheeseman

    Insert

    Bod1 (BOD1 Human)
    

    Use

    Retroviral

    Tags

    eYFP-TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

11. PP-AHA1

    Plasmid

    #28123

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PFC0360w:MAC03H-A08:C212775 (PFC0360w Plasmodium falciparum)
    

    Use

    Tags

    His tag with TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

12. PB-TAC-ERP2-(N-HA)KRAS_G12D

    Plasmid

    #201254

    Purpose

    Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

    Depositor

    Alexander Kleger

    Article

    Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

    Insert

    KRAS (KRAS Human)
    

    Use

    Piggybac transposon

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    changed Gly (G) 12 to Asp (D)

    Promoter

    TRE

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKG123

    Plasmid

    #63074

    Purpose

    Retroviral expression of mCherry-TEV-S-tag CENP-C for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    CENP-C (CENPC Human)
    

    Use

    Retroviral

    Tags

    TEV-S and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR1A-Huwe1

    Plasmid

    #37431

    Depositor

    Jean Cook

    Insert

    Huwe1 (HUWE1 Human)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid

    #196712

    Purpose

    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-KRAB(ZIM3)-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. LO601: pMVP (R4-R3) destabilized firefly luciferase (Luc2P)

    Plasmid

    #132926

    Purpose

    pMVP R4-R3 entry plasmid, contains destabilized Firefly Luciferase (Luc2P) for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Insert

    Luc2P
    

    Use

    Luciferase and Synthetic Biology; Pmvp gateway en…

    Tags

    PEST destabilization domain

    Expression

    Mutation

    Promoter

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-DIO-CAG-hM3Dq-P2A-dTomato

    Plasmid

    #202036

    Purpose

    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM3Dq-2A-dTomato

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Insert

    hM3Dq
    

    Use

    AAV

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDSG594

    Plasmid

    #214221

    Purpose

    competition strain 10 integrase plasmid

    Depositor

    Uri Alon

    Article

    Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

    Insert

    PhlF, A118
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-DIO-CAG-hM4Di-P2A-dTomato

    Plasmid

    #202037

    Purpose

    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomato

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Insert

    hM4Di
    

    Use

    AAV

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-FLEX-SaCas9-U6-sgNts

    Plasmid

    #159909

    Purpose

    Mutagenesis of Nts

    Depositor

    Larry Zweifel

    Insert

    Nts (Nts Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAP1-3

    Plasmid

    #71258

    Purpose

    luciferase reporter with 3 copies of the stromelylisin gene AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

    Depositor

    Peter Cockerill

    Insert

    3 copies of an AP-1 site from the human Stromelysin-1 gene (MMP3 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GM-CSF minimal

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET15b His6-ecDHFR (WT)

    Plasmid

    #73188

    Purpose

    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)

    Depositor

    Thomas Wandless

    Insert

    folA dihydrofolate reductase (folA E. coli)
    

    Use

    Tags

    His 6X

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pAAV-CMV-FLEX-SaCas9-U6-sgVamp2

    Plasmid

    #159920

    Purpose

    Mutagenesis of Vamp2

    Depositor

    Larry Zweifel

    Insert

    Vamp2 (Vamp2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hSyn-DIO-jGCamp7b-P2A-GSG-mRuby3

    Plasmid

    #130900

    Purpose

    bicistronic plasmid for dual-color neuronal calcium and morphology imaging

    Depositor

    Simon Wiegert

    Insert

    jGCaMP7b-P2A-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pBS_flGAD

    Plasmid

    #87031

    Purpose

    Expresses GAD (Glutamic Acid Decarboxylase) in Bacteria

    Depositor

    Janice Blum

    Insert

    flGAD (GAD2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac-Z

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pmCellFree_KA1_SARS-COV-2_ORF3a

    Plasmid

    #169390

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3a with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _ORF3a (ORF3a SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

    Plasmid

    #185060

    Purpose

    Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445

    Depositor

    Bobby Koeleman

    Insert

    U6:pegRNA:scaffold:PBS+RT template
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDSG578

    Plasmid

    #214215

    Purpose

    integrase plasmid

    Depositor

    Uri Alon

    Article

    Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

    Insert

    PhlF, A118
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-CMV-FLEX-SaCas9-U6-sgSlc6a3

    Plasmid

    #159902

    Purpose

    Mutagenesis of Slc6a3

    Depositor

    Larry Zweifel

    Insert

    Slc6a3 (Slc6a3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TMPRSS2 g1 + Cas9

    Plasmid

    #153015

    Purpose

    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + TMPRSS2 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge